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A new method uses E. coli to generate DNA with methylation patterns that target microbes recognize and accept as their own, facilitating customization of microbes for biofuels production.

Scientists at the US Department of Energy’s Oak Ridge National Laboratory have demonstrated a method to insert genes into a variety of microorganisms that previously would not accept foreign DNA, with the goal of creating custom microbes to break down plants for bioenergy.

Samples of 70% dark chocolate prepared for study with the USANS instrument at the Spallation Neutron Source. (Credit: ORNL/Genevieve Martin)

Tempering, the heating process that gives chocolate its appealing sheen and creamy texture, is a crucial part of crafting quality chocolate. But, at the molecular level, it gets a little tricky, and when done incorrectly, can render entire batches of chocolate gritty and unappetizing.

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Mircea Podar has travelled around the world and to the bottom of the ocean in pursuit of scientific discoveries, but it is the uncharted territory he encounters when working with new microbes that inspires his research at ORNL.

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Working backwards has moved Josh Michener’s research far forward as he uses evolution and genetics to engineer microbes for better conversion of plants into biofuels and biochemicals. In his work for the BioEnergy Science Center at ORNL, for instance, “we’ve gotten good at engineering microbes th...