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By engineering the Serine Integrase Assisted Genome Engineering (SAGE) genetic toolkit in an industrial strain of Aspergillus niger, we have established its proof of principle for applicability in Eukaryotes.

System and method for part porosity monitoring of additively manufactured components using machining
In additive manufacturing, choice of process parameters for a given material and geometry can result in porosities in the build volume, which can result in scrap.

We present a comprehensive muti-technique approach for systematic investigation of enzymes generated by wastewater Comamonas species with hitherto unknown functionality to wards the depolymerization of plastics into bioaccessible products for bacterial metabolism.

Distortion generated during additive manufacturing of metallic components affect the build as well as the baseplate geometries. These distortions are significant enough to disqualify components for functional purposes.

The invention introduces a novel, customizable method to create, manipulate, and erase polar topological structures in ferroelectric materials using atomic force microscopy.

For additive manufacturing of large-scale parts, significant distortion can result from residual stresses during deposition and cooling. This can result in part scraps if the final part geometry is not contained in the additively manufactured preform.

High coercive fields prevalent in wurtzite ferroelectrics present a significant challenge, as they hinder efficient polarization switching, which is essential for microelectronic applications.

In additive manufacturing large stresses are induced in the build plate and part interface. A result of these stresses are deformations in the build plate and final component.

Detection of gene expression in plants is critical for understanding the molecular basis of plant physiology and plant responses to drought, stress, climate change, microbes, insects and other factors.