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Laser Capture Microdissection–liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells

by John F Cahill, Vilmos Kertesz
Publication Type
Book Chapter
Publication Date
Page Numbers
89 to 101
Publisher Name
Springer
Publisher Location
New York, New York, United States of America

Laser capture microdissection is valuable technique in individually isolating single cells whether in tissue networks or deposited from a cell suspension. New developments have enabled coupling of laser capture microdissection with mass spectrometry via liquid vortex capture sampling probe. This enables on-line metabolic profiling of sectioned cells. Here, we describe the protocol used to deposit, isolate, and individually chemically characterize single Allium cepa and Chlamydomonas reinhardtii cells by laser capture microdissection-liquid vortex capture-mass spectrometry.