Mitchel John Doktycz

Mitchel John Doktycz


Mitchel J. Doktycz is a Distinguished Staff Scientist and Leader of the Biological and Nanoscale Systems Group in the Biosciences Division at the Oak Ridge National Laboratory (ORNL). He is also a member of the Research Staff in the Center for Nanophase Materials Sciences at ORNL and has appointments at the University of Tennessee, Knoxville in the UT-ORNL Bredesen Center for Interdisciplinary Research and Graduate Education, the Genome Science and Technology Program and the Department of Chemical and Biomolecular Engineering. He received his B.S. in Biology and Chemistry in 1985 and his Ph.D. in Chemistry in 1991 from the University of Illinois at Chicago. His research interests focus on the intersection of natural and synthetic systems.  His laboratory is involved in the development of analytical technologies for post-genomics studies with specific emphases on molecular and cellular imaging techniques and the use of nanomaterials to study and mimic biological systems. He has received an R&D 100 Award and National Federal Laboratory Consortium Award for Excellence in Technology Transfer.  He is a Fellow of the American Institute for Medical and Biological Engineering.


2013    Battelle Distinguished Inventor

2008    Fellow of the American Institute for Medical and Biological Engineering

2004    Life Sciences Division Award for Scientific Achievement

2003    National Federal Laboratory Consortium Award for Excellence in Technology Transfer

2002    R&D 100 Award

2001    Southeast Region Federal Laboratory Consortium Award for Technology Transfer

1997    Lockheed Martin Energy Research Publication Award

1990    Bernard Babler Award for Excellence in Teaching, University of Illinois at Chicago

1987     Merit Award for Outstanding Teaching in Chemistry, University of Illinois at Chicago


Biosciences Division:

Plant Microbe Interfaces:


A full list of contributions can found using the ORCID ID: 0000-0003-4856-8343 or using Google Scholar (“Mitch Doktycz”)

  1. Mohr, B.P., Retterer, S.T. and Doktycz, M. J. (2016) “While-you-wait proteins? Producing biomolecules at the point of need” Expert Review of Proteomics, 13(8) 707-709.
  2. Utturkar,S. M., Cude, W. N., Robeson II, M. S., Yang, Z. K., Klingeman, D. M., Land, M. L., Allman, S. L., Lu, T.-S. Y., Brown, S. D.,Schadt, C.W., Podar, M., Doktycz, M. J., and Pelletier, D. A (2016) “Enrichment of root endophytic bacteria from Populus deltoides and single-cell genomics analysis” Applied and Environmental Microbiology, in press.
  3. Hansen, R.H., Timm, C.M., Bible, A.N., Morrell-Falvey, J.L., Pelletier, D.L., Simpson, M.L., Doktycz, M.J., Retterer, S.T. (2016) “Stochastic Assembly of Bacteria in Microwell Arrays Reveals the Importance of Confinement in Community Development.” PLOS 11(4) e0155080.
  4. Timm, C. M., Pelletier, D. A., Jawdy, S. S., Gunter, L. E., Henning, J. A., Engle, N., Aufrecht, J., Gee, E., Nookaew, I., Yang, Z., Lu, T.-Y., Tschaplinski, T. J., Doktycz, M. J., Tuskan, G. A., Weston, D. J. (2016) “Two poplar-associated bacterial isolates induce additive favorable responses in a constructed plant-microbiome system” Frontiers in Plant Science, in press.
  5. Bible, A. N., Fletcher, S. J., Pelletier, D. A., Schadt, C. W., Jawdy, S. S., Weston, D. J., Engle, N. L., Tschaplinski, T. T., Masyuko, R., Polisetti, S., Bohn, P. W., Coutinho, T. A., Doktycz, M. J., Morrell-Falvey, J. L. (2016) “A carotenoid-deficient mutant in Pantoea sp. YR343, a bacteria isolated from the rhizosphere of Populus deltoides, is defective in root colonization” Frontiers in Microbiology, in press.
  6. Jun, S.-R., Wassenaar, T.M., Nookaew, I., Hauser, L., Wanchai, V., Land, M., Timm, C., Lu, T.-Y., Schadt, C.W., Doktycz, M.J., Pelletier, D.A., Ussery, D.W. (2016) “Diversity of Pseudomonas Genomes, Including Populus-Associated Isolates, as Revealed by Comparative Genome Analysis” Applied and Environmental Microbiology, 82(1) 375-383.
  7. Timm, C., Hansen, R., Retterer, S., Doktycz, M., Pelletier, D. (2015) Microstencils to generate defined, multi-species patterns of bacteria Biomicrofluidics 9(6), Article Number :064103. 
  8. Timm, A.C., Shankles, P.G., Foster, C.M., Doktycz, M.J., Retterer, S.T., (2015) “Towards microfluidic reactors for cell-free protein synthesis at the point-of-care” Small, 12(6) 810-817.
  9. Timm, A.C., Shankles, P.G., Foster, C.M., Doktycz, M. J., Retterer, S. T., (2015) “Characterization of extended channel bioreactors for continuous-flow protein production” Journal of Vacuum Science and Technology B, 33(6) Article Number: 06FM02.
  10. Shankles, P.G., Timm, A.C., Doktycz, M.J., Retterer, S. T. (2015) “Fabrication of Nanoporous Membranes for Tuning Microbial Interactions and Biochemical Reactions” Journal of Vacuum Science and Technology B, 33(6) Article Number: 06FM03.
  11. .Millet, L. J., Doktycz, M. J., Retterer, S. T., (2015) “Nanofluidic interfaces in microfluidic networks.” Journal of Vacuum Science and Technology B 33(6) Article Number: 06FM01
  12. Timm, C.M., Campbell, A.G., Utturkar, S.M., Jun, S., Parales, R.E., Tan, W., Robeson, M.S., Lu, T.-Y., Jawdy, S., Brown, S.D., Ussery, D.W., Schadt, C.W., Tuskan, G. A., Doktycz, M.J., Weston, D.J., Pelletier, D.A. (2015) “Metabolic functions of Pseudomonas fluorescens strains from Populus deltoides depend on rhizosphere or endosphere isolation compartment” Frontiers in Microbiology, 6, Article 111
  13. Suresh, A. K., Pelletier, D. A., Moon, J.-W., Phelps, T. J., Doktycz, M. J. (2015) “Size-separation of silver nanoparticles using sucrose gradient centrifugation” J. Chromatogr. Sep. Tech.,6(5), 1000283.
  14. Millet, L J, Lucheon, J. D., Standaert, R. F., Retterer, S. T. and Doktycz, M. J. (2015) “Modular Microfluidics for Point-of-Care Protein Purifications.” Lab on a Chip, 15(8) 1799-1811.
  15. Nallathamby, P. D, Mortensen, N.P., Palko, H. A., Malfatti, M., Smith, C., Sonnett, J., Doktycz, M. J., Gu, B., Wang, W. Retterer, S. T. (2015) “New Surface Radiolabeling Schemes of Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) for Biodistribution Studies.” Nanoscale 7 (15). The Royal Society of Chemistry: 6545–55. doi:10.1039/C4NR06441K.
  16. Utturkar, S. M., Klingeman, D. M., Land, M. L., Schadt, C. W., Doktycz, M. J., Pelletier, D. A., Brown, S. D. (2014) “Evaluation and validation of de novo and hybrid assembly techniques to derive high quality genome sequences” 
doi: 10.1093/bioinformatics/btu391.
  17. Hansen, R., Shubert, K., Morrell-Falvey, J., Lokitz, B., Doktycz, M., Retterer, S. (2014) “Microstructured block copolymer surfaces for control of microbe adhesion and aggregation” Biosensors, 4, 63-75.
  18. Tschaplinski, T. J., Plett, J. M., Engle, N. L., Deveau, A., Cushman, K. C., Martin, M. Z., Doktycz, M. J., Tuskan, G. A., Brun, A., Kohler, A., Martin, F. (2014) “Populus trichocarpa and Populus deltoides exhibit different metabolomic responses to colonization by the symbiotic fungus Laccaria bicolor” Molecular Plant Microbe Interactions, 27(6) 546-556.
  19. Iyer, S., and Doktycz M. J. (2014) “Thrombin-mediated transcriptional regulation using DNA aptamers in DNA-based cell free protein synthesis” ACS Synthetic Biology,3 (6), 340–346.
  20. Iyer, S., Karig, D. K., Norred, S. E., Simpson, M. L., Doktycz, M.J. (2013) “Multi-input regulation and logic with T7 promoters in cells and cell free systems” PLoS ONE 8(10): e78442.
  21. Wang, W., Nallathamby, P.D., Foster, C.M., Morrell-Falvey, J. L., Mortensen, N. P., Doktycz, M.J., Gu, B., Retterer, S.T. (2013) “Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) Nanoparticles”, Nanoscale, 5, 10369-10375.
  22. Mortensen, N. O., Boisen, N., Trevino-Dapatka, S., Kennel, S. J., Fowlkes, J. D., Doktycz, M. J., Nataro, J. P., Allison, D. P. (2013) “Enteroaggregative Escherichia coli Surface Protein Dispersin Increases Bacterial Uptake of Ciprofloxacin” International Journal of Antimicrobial Agents, 42 462-465.
  23. Hinestrosa, J. P., Sutton, J. E., Allison, D. P., Doktycz, M. J., Messman, J. M., Retterer, S. T., (2013) “Layer-by-Layer Templated Assembly of Silica at the Nanoscale”, Langmuir, 29(7) 2193-2199. 
  24. Kumar, A., Karig, D., Acharya, R., Neethirajan, S., Mukherjee, P. P., Retterer, S., Doktycz, M. J. (2012) “Microscale confinement features can affect biofilm formation”, Microfluid Nanofluid, 14(5), 895-902.
  25. Suresh, A. K., Pelletier, D. A., and Doktycz, M. J. (2013) “Relating nanomaterial properties and microbial toxicity”, Nanoscale, 5(2), 463-474.
  26. Lian, M., Collier, C. P., Doktycz, M. J., Retterer, S. T., (2012) “Monodisperse alginate microgel formation in a three-dimensional microfluidic droplet generator”, Biomicrofluidics, 6(4) 044108.
  27. Brown, S. D., Utturkar, S.M., Klingeman, D.M., Johnson, C. M., Martin, S. L., Land, M. L., Lu, T.-Y.S. Schadt, C.W., Doktycz, M.J., and Pelletier, D.A. (2012) “Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides”, Journal of Bacteriology, 194(21) 5991-5993.
  28. Kumar, A., Karig, D., Neethirajan, S., Suresh, A. K., Srijanto, B. R., Mukherjee, P. P., Retterer, S. T., Doktycz, M.J. (2012) “Adhesion and formation of biofilms in complex microfluidic devices”, Proc ASME-MNHMT2012, Paper # MNHMT2012-75207
  29. Weston, D.J., Pelletier, D. A., Morrell-Falvey, J. L., Tschaplinski, T. J., Jawdy, S.S., Lu, T.-Y., Melton, S. J., Martin, M., Schadt, C. W., Karve, A. A., Chen, J.-G., Doktycz, M. J., Tuskan, G. A. (2012) “Pseudomonas fluorescens induces strain-dependent and strain-independent host plant responses in defense networks, primary metabolism and photosynthesis” Molecular Plant-Microbe Interactions, 25(6) 765–778.
  30. Brown, S. D., Klingeman, D. M, Lu, T.-Y. S., Johnson, C., Utturkar, S., Land, M., Schadt, C. W., Doktycz, M. J., and Pelletier, D. A. (2012) “Draft Genome Sequence of Rhizobium sp. PDO1-076, a bacterium isolated from Populus deltoides, Journal of Bacteriology, 194(9), 2383.
  31. Suresh, A. K., Pelletier, D. A., Wang, W., Morrell-Falvey, J. L., Gu, B., and Doktycz, M. J. (2012) “Cytotoxicity Induced by Engineered Silver Nanocrystallites is Dependent on Surface Coatings and Cell Types” Langmuir, 28(5) 2727-2735.
  32. Siuti, P., Retterer, S. T., Choi, C.-K., Doktycz, M. J. (2012) “Enzyme reactions in picoliter, nanoporous containers”, Analytical Chemistry, Analytical Chemistry, 84(2) 1092-1097.
  33. Karig, D. K., Iyer, S., Simpson, M. L., Doktycz, M. J. (2012) “Expression Optimization and Inducible Negative Feedback in Cell-Free Systems”, Nucleic Acids Research, 40(8) 3763-3774.
  34. Siuti, P., Green, C., Edwards, A. N., Doktycz, M. J., Alexandre, G. (2011) “Nitrogen availability modulates surface attachment of Azospirillum brasilense while changes in adhesive cell surface properties regulated by the chemotaxis-like pathway Che1 have an indirect role”, FEMS Microbiology Letters, 323(2) 105-112