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Mechanisms of Subzero Growth In the Cryophile Planococcus Halocryophilus Determined Through Proteomic Analysis

Publication Type
Journal Name
Environmental Microbiology
Publication Date
Page Numbers
4460 to 4479

This is the first whole cell proteomic study performed on a subzero-growing permafrost-associated isolate at such a low temperature (-10 °C) and one that connects subzero growth and the salt response. Through whole cell proteomic analyses at -10 °C, 23 °C, and high/low salt conditions, we could identify functional pathways and mechanisms important for subzero growth in the extremophile Planococcus halocryophilus. Our results highlight a closely tied relationship between subzero and salt responses in the organism and identify a possible new mechanism for translational reprogramming at subzero temperatures. We find evidence for a complex cell cycle response and identify candidate proteins with potentially important roles in the unique cellular membrane modifications and peptidoglycan encrustations observed at subzero temperatures in this organism.