Kevin L Weiss

Kevin L Weiss

CSMB Laboratory Manager and Technical Lead


Kevin Weiss is the Bio-Deuteration Laboratory technical lead and laboratory manager for the Center for Structural Molecular Biology laboratories at the SNS. He received his B.S. in Pharmacy from Ohio Northern University and his Ph.D. in Medicinal Chemistry from the Ohio State University. Following graduation, his research focus shifted from small molecule organic synthesis to structural biology while he held successive postdoctoral appointments at Vanderbilt University (advisor: Walter Chazin) and ORNL (advisor: Dean Myles). His current research interests are in fed-batch fermentation, protein expression and purification, and deuterium labeling for neutron structural biology.


(1) Azadmanesh, J.; Lutz, W. E.; Weiss, K. L.; Coates, L.; Borgstahl, G. E. O. Redox Manipulation of the Manganese Metal in Human Manganese Superoxide Dismutase for Neutron Diffraction. Acta Crystallogr. 2018, F74 (10), 677–687.

(2) Kovalevsky, A.; Aggarwal, M.; Velazquez, H.; Cuneo, M. J.; Blakeley, M. P.; Weiss, K. L.; Smith, J. C.; Fisher, S. Z.; McKenna, R. “To Be or Not to Be” Protonated: Atomic Details of Human Carbonic Anhydrase-Clinical Drug Complexes by Neutron Crystallography and Simulation. Structure 2018, 26 (3), 383–390.

(3) Langan, P. S.; Vandavasi, V. G.; Cooper, S. J.; Weiss, K. L.; Ginell, S. L.; Parks, J. M.; Coates, L. Substrate Binding Induces Conformational Changes in a Class A β-Lactamase That Prime It for Catalysis. ACS Catal. 2018, 8 (3), 2428–2437.

(4) Banks, A.; Qin, S.; Weiss, K. L.; Stanley, C. B.; Zhou, H.-X. Intrinsically Disordered Protein Exhibits Both Compaction and Expansion under Macromolecular Crowding. Biophys. J. 2018, 114 (5), 1067–1079.

(5) Naing, S.-H.; Oliver, R. C.; Weiss, K. L.; Urban, V. S.; Lieberman, R. L. Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering. Biophys. J. 2018, 114 (3), 602–608.

(6) Dajnowicz, S.; Johnston, R. C.; Parks, J. M.; Blakeley, M. P.; Keen, D. A.; Weiss, K. L.; Gerlits, O.; Kovalevsky, A.; Mueser, T. C. Direct Visualization of Critical Hydrogen Atoms in a Pyridoxal 5’-Phosphate Enzyme. Nat. Commun. 2017, 8 (1), 955.

(7) Azadmanesh, J.; Trickel, S. R.; Weiss, K. L.; Coates, L.; Borgstahl, G. E. O. Preliminary Neutron Diffraction Analysis of Challenging Human Manganese Superoxide Dismutase Crystals. Acta Crystallogr. 2017, F73 (4), 235–240.

(8) O’Dell, W. B.; Swartz, P. D.; Weiss, K. L.; Meilleur, F. Crystallization of a Fungal Lytic Polysaccharide Monooxygenase Expressed from Glycoengineered Pichia Pastoris for X-Ray and Neutron Diffraction. Acta Crystallogr. 2017, F73 (2), 70–78.

(9) Vandavasi, V. G.; Langan, P. S.; Weiss, K. L.; Parks, J. M.; Cooper, J. B.; Ginell, S. L.; Coates, L. Active-Site Protonation States in an Acyl-Enzyme Intermediate of a Class A β-Lactamase with a Monobactam Substrate. Antimicrob. Agents Chemother 2017, 61 (1).

(10) Langan, P. S.; Vandavasi, V. G.; Weiss, K. L.; Cooper, J. B.; Ginell, S. L.; Coates, L. The Structure of Toho1 β-Lactamase in Complex with Penicillin Reveals the Role of Tyr105 in Substrate Recognition. FEBS Open Bio 2016, 6 (12), 1170–1177.

(11) Gerlits, O.; Wymore, T.; Das, A.; Shen, C.-H.; Parks, J. M.; Smith, J. C.; Weiss, K. L.; Keen, D. A.; Blakeley, M. P.; Louis, J. M.; et al. Long-Range Electrostatics-Induced Two-Proton Transfer Captured by Neutron Crystallography in an Enzyme Catalytic Site. Angew. Chem. 2016, 55 (16), 4924–4927.

(12) McFeeters, H.; Vandavasi, V. G.; Weiss, K. L.; Coates, L.; McFeeters, R. L. Neutron Diffraction Analysis of Pseudomonas Aeruginosa Peptidyl-TRNA Hydrolase 1. Acta Crystallogr. 2016, F72 (3), 220–223.

(13) Knihtila, R.; Holzapfel, G.; Weiss, K.; Meilleur, F.; Mattos, C. Neutron Crystal Structure of RAS GTPase Puts in Question the Protonation State of the GTP γ-Phosphate. J. Biol. Chem. 2015, 290 (52), 31025–31036.

(14) Coates, L.; Cuneo, M. J.; Frost, M. J.; He, J.; Weiss, K. L.; Tomanicek, S. J.; McFeeters, H.; Vandavasi, V. G.; Langan, P.; Iverson, E. B. The Macromolecular Neutron Diffractometer MaNDi at the Spallation Neutron Source. J. Appl. Crystallogr. 2015, 48 (4), 1302–1306.

(15) Wan, Q.; Zhang, Q.; Hamilton-Brehm, S.; Weiss, K.; Mustyakimov, M.; Coates, L.; Langan, P.; Graham, D.; Kovalevsky, A. X-Ray Crystallographic Studies of Family 11 Xylanase Michaelis and Product Complexes: Implications for the Catalytic Mechanism. Acta Crystallogr. 2014, D70 (Pt 1), 11–23.

(16) Heller, W. T.; Urban, V. S.; Lynn, G. W.; Weiss, K. L.; O’Neill, H. M.; Pingali, S. V.; Qian, S.; Littrell, K. C.; Melnichenko, Y. B.; Buchanan, M. V.; et al. The Bio-SANS Instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory. Journal of Applied Crystallography 2014, 47 (4), 1238–1246.

(17) Coates, L.; Tomanicek, S.; Schrader, T. E.; Weiss, K. L.; Ng, J. D.; Jüttner, P.; Ostermann, A. Cryogenic Neutron Protein Crystallography: Routine Methods and Potential Benefits. Journal of Applied Crystallography 2014, 47 (4), 1431–1434.

(18) Wan, Q.; Kovalevsky, A.; Zhang, Q.; Hamilton-Brehm, S.; Upton, R.; Weiss, K. L.; Mustyakimov, M.; Graham, D.; Coates, L.; Langan, P. Heterologous Expression, Purification, Crystallization and Preliminary X-Ray Analysis of Trichoderma Reesei Xylanase II and Four Variants. Acta Crystallogr. 2013, F69 (3), 320–323.

(19) Tomanicek, S. J.; Standaert, R. F.; Weiss, K. L.; Ostermann, A.; Schrader, T. E.; Ng, J. D.; Coates, L. Neutron and X-Ray Crystal Structures of a Perdeuterated Enzyme Inhibitor Complex Reveal the Catalytic Proton Network of the Toho-1 Beta-Lactamase for the Acylation Reaction. J Biol Chem 2013, 288 (7), 4715–4722.

(20) Nickels, J. D.; O’Neill, H.; Hong, L.; Tyagi, M.; Ehlers, G.; Weiss, K. L.; Zhang, Q.; Yi, Z.; Mamontov, E.; Smith, J. C.; et al. Dynamics of Protein and Its Hydration Water: Neutron Scattering Studies on Fully Deuterated GFP. Biophys J 2012, 103 (7), 1566–1575.

(21) Chu, X.; Gajapathy, M.; Weiss, K. L.; Mamontov, E.; Ng, J. D.; Coates, L. Dynamic Behavior of Oligomeric Inorganic Pyrophosphatase Explored by Quasielastic Neutron Scattering. J Phys Chem B 2012, 116 (33), 9917–9921.

(22) Clifton, L. A.; Johnson, C. L.; Solovyova, A. S.; Callow, P.; Weiss, K. L.; Ridley, H.; Le Brun, A. P.; Kinane, C. J.; Webster, J. R. P.; Holt, S. A.; et al. Low Resolution Structure and Dynamics of a Colicin-Receptor Complex Determined by Neutron Scattering. J Biol Chem 2012, 287 (1), 337–346.

(23) Munshi, P.; Chung, S.-L.; Blakeley, M. P.; Weiss, K. L.; Myles, D. A. A.; Meilleur, F. Rapid Visualization of Hydrogen Positions in Protein Neutron Crystallographic Structures. Acta Crystallogr. 2012, D68 (Pt 1), 35–41.

(24) Borreguero, J. M.; He, J.; Meilleur, F.; Weiss, K. L.; Brown, C. M.; Myles, D. A.; Herwig, K. W.; Agarwal, P. K. Redox-Promoting Protein Motions in Rubredoxin. J Phys Chem B 2011, 115 (28), 8925–8936.

(25) Tomanicek, S. J.; Wang, K. K.; Weiss, K. L.; Blakeley, M. P.; Cooper, J.; Chen, Y.; Coates, L. The Active Site Protonation States of Perdeuterated Toho-1 β-Lactamase Determined by Neutron Diffraction Support a Role for Glu166 as the General Base in Acylation. FEBS Lett 2011, 585 (2), 364–368.

(26) Gardberg, A. S.; Del Castillo, A. R.; Weiss, K. L.; Meilleur, F.; Blakeley, M. P.; Myles, D. A. A. Unambiguous Determination of H-Atom Positions: Comparing Results from Neutron and High-Resolution X-Ray Crystallography. Acta Crystallogr. 2010, D66 (5), 558–567.

(27) Meilleur, F.; Weiss, K. L.; Myles, D. A. A. Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis. In Methods in Molecular Biology; Lee, J. W., Foote, R. S., Eds.; Micro and Nano Technologies in Bioanalysis: Methods and Protocols; 2009; Vol. 544, pp 281–292.

(28) Weiss, K. L.; Meilleur, F.; Blakeley, M. P.; Myles, D. A. Preliminary Neutron Crystallographic Analysis of Selectively CH3-Protonated Deuterated Rubredoxin from Pyrococcus Furiosus. Acta Crystallogr. 2008, F64 (6), 537–540.

(29) Teixeira, S. C. M.; Zaccai, G.; Ankner, J.; Bellissent-Funel, M. C.; Bewley, R.; Blakeley, M. P.; Callow, P.; Coates, L.; Dahint, R.; Dalgliesh, R.; et al. New Sources and Instrumentation for Neutrons in Biology. Chemical Physics 2008, 345 (2–3), 133–151.

(30) Meyn, S. M.; Seda, C.; Campbell, M.; Weiss, K. L.; Hu, H.; Pastrana-Rios, B.; Chazin, W. J. The Biochemical Effect of Ser167 Phosphorylation on Chlamydomonas Reinhardtii Centrin. Biochem. Biophys. Res. Commun. 2006, 342 (1), 342–348.

(31) Shah, V. N.; Wingo, T. L.; Weiss, K. L.; Williams, C. K.; Balser, J. R.; Chazin, W. J. Calcium-Dependent Regulation of the Voltage-Gated Sodium Channel HH1: Intrinsic and Extrinsic Sensors Use a Common Molecular Switch. Proc Natl Acad Sci U S A 2006, 103 (10), 3592–3597.

(32) Chapman, J. S.; Weiss, K. L.; Curley, R. W. J.; Highland, M. A.; Clagett-Dame, M. Hydrolysis of 4-HPR to AtRA Occurs in Vivo but Is Not Required for Retinamide-Induced Apoptosis. Arch. Biochem. Biophys. 2003, 419 (2), 234–243.

(33) Weiss, K. L.; Alshafie, G.; Chapman, J. S.; Mershon, S. M.; Abou-Issa, H.; Clagett-Dame, M.; Curley, R. W. An Unhydrolyzable Analogue of N-(4-Hydroxyphenyl)Retinamide: Synthesis and Preliminary Biological Studies. Bioorg. Med. Chem. Lett. 2001, 4.

(34) Abou-Issa, H.; Curley Jr., R. W.; Alshafie, G. A.; Weiss, K. L.; Clagett-Dame, M.; Chapman, J. S.; Mershon, S. M. Chemotherapeutic Evaluation of 4-Hydroxybenzylretinone (4-HBR), a Nonhydrolyzable c-Linked Analog of n-(4-Hydroxyphenyl) Retinamide (4-HPR) against Mammary Carcinogenesis. Anticancer Research 2001, 21 (6 A), 3839–3844.

(35) Wong, M. F.; Weiss, K. L.; Curley, R. W. Recent Improvements towards the Synthesis of the C-Glucuronosyl Cancer Chemopreventive (Beta-D-Glucopyranosyluronate)-4-Retinamidophenylmethane. Journal of Carbohydrate Chemistry 1996, 15 (6), 763–768.

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