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Barbara R Evans

Barbara R Evans

Bio

Barbara R. Evans Curriculum Vitae

Business Address

Oak Ridge National Laboratory
4100 Bethel Valley Road
Oak Ridge, TN 37831-6106
Tel. 865-241-3185
E-mail evansb@ornl.gov

Education
1979 B. A. Chemistry, College of St. Catherine, St. Paul, MN
1979 B. A. German, College of St. Catherine, St. Paul, MN
1986 Ph.D. Chemistry, University of California, San Diego, CA

Scholarships and Organizations
1994-Present American Chemical Society
1992-Present Archeological Institute of America
1981 Present American Association for the Advancement of Science
1979 1980 Fulbright Scholarship (West Germany)
1979 Phi Beta Kappa
1975 1979 National Merit Scholarship

Research and Professional Experience
1999-Present Staff Scientist, Oak Ridge National Laboratory, Oak Ridge, TN
1995-1999 Senior Biochemist, Kelly Scientific Resources/Oak Ridge Research Institute, Oak Ridge, TN
1992-1995 Postdoctoral Fellow, Oak Ridge National Laboratory, Oak Ridge, TN
1990 1991 Postdoctoral Fellow, Department of Microbiology, University of Texas, Austin, TX
1987 1989 Postdoctoral Fellow, Scripps Clinic and Research Foundation, La Jolla, CA

Publications

1. Lee, J. W., Hawkins, B., Kidder, M. K., Evans, B. R., Buchanan, A. C., Day, D. “Characterization of Biochars Produced from Peanut Hulls and Pine Wood with Different Pyrolysis Conditions.” (2016) Bioresources and Bioprocessing 3(1): article 15.

2. O’Neill, H., Shah, R., Evans, B., He, J., Pingali, S. V., Chundawat, S. P. S., Jones, A. D., Langan, P., Davison, B. H., Urban, V., “Production of Bacterial Cellulose with Controlled Deuterium-Hydrogen Substitution for Neutron Scattering Studies” in Methods in Enzymology: Volume 565 Isotope Labeling of Biomolecules, ed. Kelman, Z., Volume 565, Chapter 6, Elsevier Ltd., Oxford, Great Britain (2015), pp. 123 – 146.

3. Evans, B. R., & Shah, R., “Development of Approaches for Deuterium Labeling in Plants”, in Methods in Enzymology: Volume 565 Isotope Labeling of Biomolecules, ed. Kelman, Z., Chapter 10, Elsevier Ltd., Oxford, Great Britain (2015), pp. 213 -243.

4. Lee, I., Evans, B. R., Bali, G., Foston, M., Ragauskas, A. J., “Silicon cantilever functionalization for cellulose-specific chemical force imaging of switchgrass.” (2015) Analytical Methods 7, 4541 - 4545.

5. Evans, B. R., Bali, G., Foston, M., Ragauskas, A. J., O’Neill, H., Shah, R., McGaughey, J., Reeves, D., Rempe, C. S., Davison, B. H. “Production of deuterated switchgrass by hydroponic cultivation,” (2015) Planta 242, 215 - 222.

6. Wang, H., Gurau, G., Pingali, S. V., O'Neill, H., Evans, B., Urban, V., Heller, W., Rogers, R., "Physical insight into switchgrass dissolution in the ionic liquid 1-ethyl-3-methylimidazolium acetate", (2014) ACS Sustainable Chemistry & Engineering (April 13, 2014) 1264-1269.

7. Evans, B., Bali, G., Reeves, D., O'Neill, H., Sun, Q., Shah, R., Ragauskas, A., “Effect of D2O on growth properties and chemical structure of annual ryegrass (Lolium multiflorum)." (2014) Journal of Agricultural and Food Chemistry 62(12), 2592 - 2604.

8. Langan, P., Petridis, L., O’Neill, H. M., Pingali, S. V., Foston, M., Nishiyama, Y., Schulz, R., Lindner, B., Hanson, B. L., Harton, S., Heller, W. T., Urban, V., Evans, b. R., Gnanakaran, S., Ragauskas, A. J., Smith, J. C., Davison, B. H. “Common Processes Drive the Thermochemical Pretreatment of Lignocellulosic Biomass.” (2014) Green Chem. 16 (1), 63-69.

9. He, J., Pingali, S. V., Chundawat, S. P. S., Pack, A., Jones, A. D., Langan, P., Davison, B. H., Urban, V., Evans, B. R., O’Neill, H. “Controlled incorporation of deuterium into bacterial cellulose.” (2014) Cellulose 21(2), 927- 936.

10. Bali, G., Foston, M. B., O’Neill, H. M., Evans, B. R., He, J., Ragauskas, A. J., “The effect of deuterium incorporation on the structure of bacterial cellulose.” (2013) Carbohydrate Res. 374, 82-88.

11. Langan, P., Evans, B. R., Foston, M., Heller, W. T., O’Neill, H. M., Petridis, L., Pingali, S. V., Ragauskas, A. J., Smith, J. C., Davison, B. “Neutron Technologies for Bioenergy Research.” (2012) Industrial Biotechnology 8(4), 209 - 216.

12. Lee, J. W., Buchanan, A. C. III, Evans, B. R., Kidder, M. (2012) Chapter 4. Oxygenation of biochar for enhanced cation exchange capacity. In Advanced Biofuels and Bioproducts, Springer Science + Business Media (New York).

13. Foston, M.B., McGaughey, J., O’Neill, H., Evans, B.R., Ragauskas, A.J. “Deuterium Incorporation in Biomass Cell Wall Components by NMR Analysis.” (2012) Analyst 137, 1090 – 1093.

14. Pingali, S.V., O'Neill, H. M., McGaughey, J., Urban, V. S., Rempe, C., Petridis, L., Smith, J. C., Evans, B. R., and Heller, W. T. “Small-angle Neutron Scattering Reveals a pH-dependent Conformational Change in Trichoderma reesei Cellobiohydrolase I: Implications for Enzymatic Activity,” (2011) J. Biol. Chem. 286 (37), 32801-32809.

15. Greenbaum, E., Evans, B. R., Chapter 10, “Synthetic Chromophores and Neural Stimulation of the Visual System” In Visual Prosthetics: Physiology, Bioengineering, Rehabilitation, ed. G. Dagnelie, Springer Science + Business Media (2011).

16. Lee, J. W., Kidder, M., Evans, B. R., Paik, S., Buchanan III, A. C., Garten, C. T., Brown, R. C. “Characterization of biochars produced from cornstovers for soil amendment and carbon sequestration.” (2010) Environmental Science and Technology 44, 7970-7974.

17. Pingali, S. V., Urban, V. S., Heller, W. T., McGaughey, J., O'Neill, H. M., Foston, M., Myles, D. A., Ragauskas, A. J., Evans, B. R. "SANS study of cellulose extracted from switchgrass" (2010) Acta Crystallographica D D66, 1189-1193.

18. Pingali, S. V., Urban, V. S., Heller, W. T., McGaughey, J., O'Neill, H. M., Foston, M., Myles, D. A., Ragauskas, A., Evans, B. R. (2010) Biomacromolecules 11, 2329-2335, "Breakdown of cell wall nanostructure in dilute acid pretreated biomass"

19. Hutchens, S. A., Benson, R. S., Evans, B. R., Rawn, C. J., O’Neill, H. M., “A resorbable calcium-deficient hydroxyapatite hydrogel composite for osseous regeneration,” (2009) Cellulose 16(5), 887-898.

20. Greenbaum, E., Humayun, M. S., Sanders, C. A., Close, D., O’Neill, H. M., Evans, B. R., “Metabolic Prosthesis for Oxygenation of Ischemic Tissue,” IEEE Trans. Biomed. Engin, 56, 528-531 (2009).

21. Ye, X., Wang, Y., Hopkins, R. C., Adams M. W., Evans B. R., Mielenz J.R., Zhang Y. H., “Spontaneous high-yield production of hydrogen from cellulosic materials and water catalyzed by enzyme cocktails.” (2009) ChemSusChem. 2(2), 149-152.

22. Zhang, Y.-H.P., Evans B. R., Mielenz, J. R., Hopkins, R.C., Adams, M. W. W., “High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway.” (2007) PLoS ONE 2(5), 456e.

23. Hutchens, S. A., León, R. V., O’Neill, H. M., Evans, B. R., “Statistical analysis of optimal culture conditions for Gluconacetobacter hansenii cellulose production.” (2007) Lett. Appl. Microbiol. 44, 175-180.

24. Hutchens, S. A., Benson, R. S., Evans, B. R., O’Neill, H. M., Rawn, C. J. “Biomimetic Synthesis and Characterization of Calcium-Deficient Hydroxyapatite in a Natural Hydrogel.” (2006) Biomaterials 27, 4661-4670.

25. Evans, B. R., O'Neill, H. M., Howe, J. Y., Greenbaum, E.,"Photocatalyzed electron transfer from Spinach PSI to metal nanoparticles." (2005) Preprint, Fuels Division, 230th National Meeting of the American Chemical Society.

26. O'Neill, H. M., Evans, B. R., Greenbaum, E., "Photodependent hydrogen evolution by Photosystem I entrapped in hybrid organo-silicate glasses." (2005) Preprint, Fuels Division, 230th National Meeting of the American Chemical Society.
27. Evans, B. R., O’Neill, H. M. “Effect of surface attachment on synthesis of bacterial cellulose.” (2005) Appl Biochem. Biotechnol. 121-124, 439-450.

28. Evans, B. R., O’Neill, H. M., Hutchens, S. A., Bruce, B. D., Greenbaum, E.. “Enhanced Photocatalytic Hydrogen Evolution by Covalent Attachment of Plastocyanin to Photosystem I.” (2004) NanoLett 4(10), 1815-1819.

29. Martin, M., Evans, B., O’Neill, H., and Woodward, J. “Laser-Induced Breakdown Spectroscopy used to Detect Palladium and Silver Metal Dispersed in Bacterial Cellulose Membranes.” (2003) Appl. Optics 42(30), 6174-6178.

30. Evans, B. R., O’Neill, H. M., Malyvanh, V. P., Lee, I. Woodward, J. “Palladium-bacterial cellulose membranes for fuel cells”, (2003) Biosens. Bioelectron. 18(7), 917-923.

31. O’Neill, H. M., Angley, C. V., Hemery, I., Evans, B. R., Dai, S., and Woodward, J., “Properties of carbohydrate-metabolizing enzymes immobilized in sol-gel beads: stabilization of invertase and –glucosidase by Blue Dextran”, (2002) Biotechnol. Lett. 24, 783-790.

32. Evans, B. R., and Woodward, J., “Protein Injury: Prevention by Antioxidants”, Chapter 3, in Biomolecular Free Radical Toxicity: Causes and Prevention, eds. H. Wiseman, P. Goldfarb, T. Ridgeway, and A. Wiseman, John Wiley and Sons, Ltd., Chichester, England, pp. 73-99 (2000).

33. Evans, B. R., Gilman, A. K., Cordray, K., and Woodward, J., “Mechanism of substrate hydrolysis by thermophilic endoglucanase from Thermotoga maritima.” (2000) Biotechnol. Lett. 22, 735-740.

34. Lee, I., Evans, B. R., and Woodward, J., “The mechanism of cellulase action on cotton fibers: Evidence from atomic force microscopy.” (2000) Ultramicroscopy 82, 213-221.

35. Woodward, J., Cordray, K. A., Edmonston, R. J., Blanco-Rivera, M., Mattingly, S. M., and Evans, B. R., “Enzymatic Hydrogen Production: Conversion of Renewable Resources for Energy Production.” (2000) Energy & Fuels 14, 197-201.

36. Evans, B. R., Lee, I., Woodward, J., and Fox, S. V., "The Effect of Cellulases on the Biodegradation and Morphology of Naturally Colored Cotton Fibers", Chapter 19, Enzyme Applications in Fiber Procesing (Eds. K.-E. L. Eriksson and A. Cavaco-Paulo), ACS Symposium Series, ACS, Washington, D. C., (1998) 228-245.

37. Woodward, J., and Evans, B. R., "Utilization of Biocatalysts in Cellulose Waste Minimization", Proceedings of EB96: Environmental Biomonitoring: The Biotechnology-Ecotoxicology Interface, Biotechnology Research Series 7, Cambridge University Press, Cambridge, U. K., (1997), 157-179.

38. Evans, B. R., Lane, L. M., Margalit, R., Hathaway, G. M., Ragauskas, A., and Woodward, J., "Comparison of the properties of native and pentaammineruthenium(III)-modified xylanase" (1996) Enzyme Microbial Technol. 19, 367-373.

39. Lee, I., Evans, B. R., Lane, L. M., and Woodward, J., "Substrate-enzyme interactions in cellulase systems", (1996) Bioresource Technol. 58, 163-169.

40. Lassig, J. P., Shultz, M. D., Gooch, M. G., Evans, B. R., and Woodward, J., “Inhibition of Cellobiohydrolase I from Trichoderma reesei by Palladium” (1995) Arch. Biochem. Biophys. 322, 119-126.

41. Shultz, M. D., Lassig, J. P., Gooch, M. G., Evans, B. R., and Woodward, J., "Palladium--a new inhibitor of cellulase activity", (1995) Biochem. Biophys. Res. Comm. 209, 1046-1052.

42. Evans, B. R., Margalit, R., and Woodward, J., "Enhanced Hydrolysis of Soluble Cellulosic Substrates by a Metallocellulase with Veratryl Alcohol Oxidase Activity", (1995) Appl. Biochem. Biotechnol. 51/52, 225-239.

43. Evans, B. R., Margalit, R., and Woodward, J., "Veratryl Alcohol Oxidase Activity of a Chemically Modified Cellulase Protein", (1994) Arch. Biochem. Biophys. 312, 459-466.

44. Woodward, J., Brown, J. P., Evans, B. R., and Affholter, K. A., "Papain Digestion of Crude Trichoderma reesei Cellulase: Purification and Properties of Cellobiohydrolase I and II Core Proteins", (1994) Biotechnol. Appl. Biochem. 19, 141-153.

45. Donner, T. R., Evans, B. R., Affholter, K. A., and Woodward, J., "The Role of the Cellulose-Binding Domain of Cellobiohydrolase I in Cellulose Hydrolysis" in Bioconversion of Fuels (Eds. M. E. Himmel and J. O. Baker) ACS Symposium Series, ACS, Washington, D. C. (1994), pp. 75-83.

46. Evans, B. R., Margalit, R., and Woodward, J., "Attachment of Pentaammine Ruthenium (III) to Trichoderma reesei Cellobiohydrolase I Increases Its Catalytic Activity", (1993) Biochem. Biophys. Res. Comm. 195, 497-503.

47. Woodward, J., Tate, J., Herrmann, P.C., and Evans, B.R., "Comparison of Ellman's Reagent with N-(1-Pyrenyl)-Maleimide for the Determination of Free Sulfhydryl Groups in Reduced Cellobiohydrolase I from Trichoderma reesei", (1993) J. Biophys. Methods 26, 121-129.

48. Chen, J.-W., Evans, B. R., Yang, S.-H., Araki, H., Oshima, Y., and Jayaram, M., "Functional Analysis of Box I Mutations in Yeast Site-Specific Recombinases Flp and R: Pairwise Complementation with Recombinase Variants Lacking the Active-Site Tyrosine", (1992) Mol. Cell. Biol. 12, 3757 - 3765.

49. Chen, J.-W., Evans, B. R., Rosenfeldt, H., and Jayaram, M., "Bending-Incompetent Variants of Flp Recombinase Mediate Strand Transfer in Half-Site Recombinations: Role of DNA Bending in Recombination", (1992) Gene 119, 37-

50. Araki, H., Nakanishi, N., Evans, B. R., Matsuzaki, H., Jayaram, M., and Oshima, Y., "Site-Specific Recombinase, R, Encoded by Yeast Plasmid pSR1", (1992) J. Mol. Biol. 225, 25-37.

51. Serre, M. C., Evans, B. R., Araki, H., Oshima, Y., and Jayaram, M., "Half-Site Recombinations Mediated by Yeast Site-Specific Recombinases Flp and R", (1992) J. Mol. Biol. 225, 621-642.

52. Chen, J.-W., Evans, B. R., Yang, S. H., Teplow, D. B., and Jayaram, M., "Domain of a Yeast Site-Specific Recombinase (Flp) that Recognizes Its Target Site", (1991) Proc. Natl. Acad. Sci. USA 88, 5944-5948.

53. Chen, J. W., Evans, B. R., Lei Zheng and Jayaram, M.,"Tyr 60 Variants of FLP Generate Conformationally Altered Protein DNA Complexes: Differential Activity in Full site and Half site Recombination", (1991) J. Mol. Biol. 218, 107 118.

54. Evans, B. R., Chen, J. W., Parsons, R. L., Bauer, T. K., Teplow, D. B. and Jayaram, M., "Identification of the Active Site Tyrosine of FLP Recombinase: Possible Relevance of Its Location to the Mechanism of Recombination", (1990) J. Biol. Chem. 265, 18504 18510.

55. Parsons, R. L., Evans, B. R., Zheng Lei and Jayaram, M. "Functional Analysis of Arg 308 Mutants of Flp Recombinase: Possible Role of Arg 308 in Coupling Substrate Binding to Catalysis", (1990) J. Biol. Chem. 265, 4527 4533.

56. Strong, D. D., Moore, M. M., Cottrell, B. A., Bohonus, V. L., Pontes, M., Evans, B. and Doolittle, R. F., "Lamprey Fibrinogen  Chain: Cloning, cDNA Sequencing, and General Characterization", (1985) Biochemistry 24, 92 102.

Patents

“Method of Tissue Repair Using a Composite Material”, Stacy A. Hutchens, Barbara R. Evans, Hugh M. O’Neill, and Jonathan Woodward. U.S. Patent No. 8,673,337 (March 18, 2014)

“High Throughput Reproducible Cantilever Functionalization”, Barbara R. Evans and Ida Lee. U.S. Patent No. 8,898,810 (November 25, 2014)

“Biochar Production Method and Composition Therefrom“, J. W. Lee, A. C. Buchanan III, B.R. Evans, M. Kidder U. S. Patent 8,398,738 (March 19, 2013).

“Composite Material”, S. A. Hutchens, J. Woodward, B. R. Evans, H. M. O’Neill. U.S. Patent
8,110,222 (February 7, 2012).

“Metallization of Bacterial Cellulose for Electrical and Electronic Device Manufacture” B. R. Evans, H. M. O’Neill, J. Woodward, V. P. Malyvanh. U. S. Patent 7,955,759 (June 7, 2011).

"Method of forming an electrically conductive cellulose composite", B. R. Evans, H. M. O’Neill, J. Woodward. U. S. Patent 8,062,868 (November 22, 2011).

Contact Information

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Publications

Production of deuterated switchgrass by hydroponic cultivation
Silicon cantilever functionalization for cellulose-specific chemical force imaging of switchgrass