Skip to main content
SHARE
Publication

One-pot generation of lignin microspheres and digestible substrate with a polyol-DES pretreatment in high solid loading

by Xianzhi Meng, Arthur J Ragauskas
Publication Type
Journal
Journal Name
Journal of Cleaner Production
Publication Date
Page Number
136322
Volume
394
Issue
1

Previous lignin microspheres (LMS) preparation needs multiple steps with very low yield and high cost. Herein, we developed a high-solid deep eutectic solvent (DES) pretreatment for an effective lignin fractionation and enzymatic saccharification of moso bamboo under mild temperature (110 °C) with high-solid loading. Lignin was significantly removed from the plant cell wall, and cellulose properties (e.g., crystallinity and degree of polymerization) were also altered during the pretreatment. As a result, the enzymatic digestibility of the pretreated bamboo was dramatically increased. Uniform micro-spherical lignin was directly produced from the pretreatment system, and its particle size could be regulated by controlling the solid loadings and pretreatment temperatures. The lignin microspheres formation mechanism was investigated by analyzing the lignin's size distribution, molecular weight distribution, chemical structure, and hydrophobicity. The DES showed excellent recyclability, and the recycled DES could still remove 42.78% lignin even after 7th circulation associated with 100% glucan saccharification. The mass balance based on 1000 g biomass showed that 196.28 g LMS was directly recovered, which exhibited a high RhB adsorption. Besides, 404.91 g glucose and 36.67 g xylose were obtained after the enzymatic saccharification process. Specifically, GAPI analysis exhibited a near total green and yellow portions of the pictogram, indicating our DES process was green enough to make this biorefinery sustainable. Overall, the proposed DES generated synergistic productions of digestible solid and LMS which could contribute to establish a green and sustainable biorefinery sequence with diverse outputs in one pot.