Getting Started
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Video Tutorials
First time conducting a remote neutron experiment? This video tutorial explains how to start a remote user session and open Control System Studio (CSS) for the first time.
Learn how to obtain a low-resolution ab initio model and structural details of a detergent-solubilized membrane protein in solution using SANS with contrast-matching of the detergent.
New to using the Biological Small-Angle Neutron Scattering (Bio-SANS) instrument? This video tutorial demonstrates how to set up and submit panel scans for an experiment using ORNL’s Bio-SANS instrument.
Need to check the status of an experiment? This tutorial explains how to check scan status and other experimental parameters such as sample environment and instrument configuration after a panel scan has been submitted.
Learn more about data reduction on Bio-SANS? This step-by-step tutorial demonstrates how to login to the analysis server and reduce your data.
Click here for more video resources about neutron scattering capabilities at ORNL.
Video Tutorials from Annual Workshops
2022
Center for Structural Molecular Biology Overview - Hugh O’Neill
Introduction to Small-Angle Neutron Scattering - Volker Urban
Virtual tour of Bio-SANS - Luke Heroux
Bio-SANS Sample Environments - Luke Heroux
Bio-Deuteration - Kevin Weiss
Virtual tour of Biology Laboratories - Kevin Weiss / Qiu Zhang
Sample Prep and Experiment Planning - Wellington Leite / Manjula Senanayake Mudiyanselage
Experiment Setup and Data Acquisition - Sai Venkatesh Pingali
Data Analysis for Solution Scattering of Biomacromolecular Complexes - Wellington Leite
Data Analysis for Hierarchical Systems - Sai Venkatesh Pingali
Data Analysis for Solution Scattering of Biomacromolecular Complexes - Wellington Leite
Data Analysis for Hierarchical Systems - Sai Venkatesh Pingali
2023
Content coming soon.
2024
Content coming soon.
2025
Content coming soon.
Data Analysis
A comprehensive software suite used for analyzing small-angle scattering data from biological macromolecules and nanoparticles.
Developed by a team of researchers led by Dmitri Svergun at the European Molecular Biology Laboratory, the ATSAS suite is a comprehensive software suite that is widely employed to analyze small-angle neutron scattering and small-angle X-ray scattering data (SANS/SAXS) from biological macromolecules and nanoparticles.
A short list of essential data analysis tools from the ATSAS software suite is listed below:
PDB-Oriented Tools
- CRYSOL, used for computing solution X-ray scattering patterns from atomic structure and comparing/fitting these patterns to experimental data
- CRYSON, used to compute/fit neutron scattering patterns
Data Processing Tools
- PRIMUS, a graphical user interface for manipulations and primary analysis of experimental one-dimensional small-angle scattering (SAS) data
- GNOM, an indirect transform program that evaluates the pair distance distribution function p(r)
Ab initio Methods
- DAMMIN, uses a “dummy atom” model for ab initio shape determination
- DAMMIF, rapid shape determination
- GASBOR, reconstructs protein structures using a chain-line ensemble of dummy residues
Rigid Body Modeling
- SASREF, for modeling of multi-subunit complexes
- BUNCH, for modeling of multi-domain proteins against multiple data sets