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Exceptional Chromosome Regions II |
Cliff Han and Norman Doggett
Los Alamos National Laboratory
825 Unigenes from chromosome 16 were BLAST against the draft and finished contigs of this chromosome. 41 unigene clusters (5%) did not hit with a similar genomic sequence using a cut-off of >97% identity and >70 bp in length. These 41 sequence were then searched against the whole human genome draft and finished contigs, and 7 of these are located on other chromosomes (1, 14, 5, 17, 19). Of the remaining 34 Unigene clusters without chromosome 16 genomic sequence one is LTR repeat, and two have poor sequence quality leaving 31 clusters for use in BAC library screening. The resulting numbers (784/815 Unigene clusters which match chromosome 16 genomic sequence) suggest that there is 96.2% coverage of the chromosome represented in the draft and finished public databases. We have selected PCR primers for the 31 Unigenes that have potential to close gaps in the 16 map and will use these to screen the RPCI-11 library. This primer list is attached.
| Hs.1137.LEFT Hs.1137.RIGHT Hs.3076.LEFT Hs.3076.RIGHT Hs.3112.LEFT Hs.3112.RIGHT Hs.8040.LEFT Hs.8040.RIGHT Hs.9003.LEFT Hs.9003.RIGHT Hs.9204.LEFT Hs.9204.RIGHT Hs.9605.LEFT Hs.9605.RIGHT Hs.74170.LEFT Hs.74170.RIGHT Hs.75124.LEFT Hs.75124.RIGHT Hs.75648.LEFT Hs.75648.RIGHT Hs.91910.LEFT Hs.91910.RIGHT Hs.96023.LEFT Hs.96023.RIGHT Hs.99880.LEFT Hs.99880.RIGHT Hs.118786.LEFT Hs.118786.RIGHT Hs.122823.LEFT Hs.122823.RIGHT Hs.145271.LEFT Hs.145271.RIGHT Hs.157113.LEFT Hs.157113.RIGHT Hs.169850.LEFT Hs.169850.RIGHT Hs.183075.LEFT Hs.183075.RIGHT Hs.189762.LEFT Hs.189762.RIGHT Hs.203936.LEFT Hs.203936.RIGHT Hs.241388.LEFT Hs.241388.RIGHT Hs.250760.LEFT Hs.250760.RIGHT Hs.275462.LEFT Hs.275462.RIGHT Hs.275675.LEFT Hs.275675.RIGHT Hs.278462.LEFT Hs.278462.RIGHT Hs.331895.LEFT Hs.331895.RIGHT Hs.332417.LEFT Hs.332417.RIGHT Hs.158336.LEFT Hs.158336.RIGHT Hs.1973.LEFT Hs.1973.RIGHT Hs.76159.LEFT Hs.76159.RIGHT |
TGTTCAGCCTGCCTTCTTTT CTCTTTAAGGAGATGGGGGC TTGTTTGGGGATGAGAAAGG CCTCTTGACACCCAGCTCTC GCATCCAATTCGTGTCTGTG GAGGGGTCAGCTCTGTCTTG GTCCCTTCCCATCAGTTTGA ACACGGTTTTCATTTAGCGG GTGTTCTGTGCCTGTGTGCT GATTAAAGCCTCCCAAAGCC CACCTTCATCACCCACCTCT CAGACCGTCCACTTCCACTT AATGCACCAGGATATGGACC TGTGCTCACAGAGACAAGCG AGTGACGCGACAACAGACAC GGTTGTGAGCTATCCGTGGT GCCTCCATCCCTGATTCTAA GGGAAGGGGTTTGATCTTTC GCCCTATTCACACTCTGGGA GGAACATGAGAGGCCAAAAA TCCTTCAGGAAGCCACTCTC CATCATCACATGAACCCTGC GGACCCAATCATGAGGAAGA TGAATCTTGGGGACTTGAGG CTTTGGGCCTATAGGGGTGT TAAATTAGCGCAAAATGGGG GACATTACGCGAAGCACTCA TTTGTTCTGACTGTCGCCTG CCCCTCAGACCTCCTCATCT CTCCCTCCTTTCCTAGGTGG CTCAGGAACAAGGGGAAACA GTTCCCAAAAACCCCTTCAG GGGTGGAGTTCAGGATCAGA GTCCTGCCAAGTCATCAGGT CAAACATCAGAGCAATCCGA TAGGGGGTCGTATGATCAGG ACCTCATTTCCTCCAACGTG ATGTAGCGGAAGAAGAGCCA CACAGGTGTGGGTCTCTCAA TTTTTCCCACTGTGGTGTCA GGACCCCAACTGCTCCTG AGGAGCAGCAGCTCTTCTTG TCCAACGCTTATGGGCTATC CAGCGACCACTGGAATCATA CATCATGGCTTTGCCTCTG TGTTGGTAGGGACGGAACTC GTGGCCATTAGCTTGGTGTT TGCCTTGCACACTAAGGTCC GCCAGTCTGGACTGAGGAAA GTTCAGGGCTGTAGAGGTGG CTTCTCCTTGCCTCGAAATG AGGAGCAGCAGCTCTTCTTG AGAAGCACATGGGGGTATTG GGACCCATTGTTCTGTGCTT CAAAGAGGGGCTTAGCACAG TTCAACTTTGCAAGAGGGCT GAAGCCTCGTGCTACCCTG TAGATGTGGGAGCAGCGG CTGTGAGCACACCACTGTCC CTGTGGGTCACATACGAACG ATCCTAGTGCCCATCGTCTG GGCGACACACAAGCTAAGGT |
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