Systematic subcellular localisation and functional characterization of novel proteins identified by large-scale cDNA sequencing

Dr. Rainer Pepperkok
Meyerhofstr.1
69117 Heidelberg,
Germany
telephone: 0049 6221 387332
fax: 0049 6221 387332
email: pepperko@embl-heidelberg.de
prestype: Platform
presenter: Rainer Pepperkok

Rainer Pepperkok, Urban Liebel, Veronika E. Neubrand, Jeremy C. Simpson, Ruth Wellenreuther (2), and Stefan Wiemann(2)
Department of Cell Biology and Biophysics, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany, email : pepperko@embl-heidelberg.de
(2) Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 280/506, 69120 Heidelberg, Germany

High-throughput cDNA sequencing projects worldwide have resulted in the identification of a large number of novel genes. The characterisation of the encoded proteins currently has remained limited to bioinformatic analyses, which could make functional predictions for only about half of the cDNAs sequenced. As a first step towards a more comprehensive functional characterisation of these cDNAs we have developed and tested a strategy, which allows their systematic and fast subcellular localization [1]. Further we have automated a number of simple microscopy based assays which aim at the better understanding of the function of these cDNAs in basic cellular processes like protein secretion, cell proliferation, apoptosis and signal transduction.

1. Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S: Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Reports 2000, 1:287-292.