Spatial and temporal expression pattern of Runx3 (aml2) and Runx1 (aml1) indicates non-redundant functions during mouse embryogenesis

Yoram Groner
The Weizmann Institute Rehovot
Israel
telephone: 972-8-9343972
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email: yoram.groner@weizmann.ac.il
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presenter: Yoram Groner

Ditsa Levanon¹, Ori Brenner², Varda Negreanu¹, David Bettoun¹, Eilon Woolf¹, Raya Eilam², Joseph Lotem¹, Yoram Groner¹
Departments of ¹Molecular Genetics and ²Experimental Animals, The Weizmann Institute of Science Rehovot 76100 ISRAEL

The human RUNX3/AML2 gene belongs to the “runt domain” family of transcription factors that act as gene expression regulators in major developmental pathways. We will describe the expression pattern of Runx3 during mouse embryogenesis compared to the expression pattern of Runx1. E10.5 and E14.5 to E16.5 embryos were analyzed using both immunohistochemistry and b-galactosidase activity of targeted Runx3 and Runx1 loci. We found that Runx3 expression overlapped with that of Runx1 in the hematopoietic system, whereas in sensory ganglia, epidermal appendages, and developing skeletal elements, their expression was confined to different compartments. These data provide new insights into the function of Runx3 and Runx1 in organogenesis and support the possibility that cross regulation between them plays a role in embryogenesis.