Beyond the Identification of Transcribed
Sequences:
Functional and Expression Analysis
11th Annual Workshop
November 9-12, 2001
Washington D.C.
Tom Freeman
Microarray Programme Leader
MRC Human Genome Mapping Programme-RC
Wellcome Trust Genome Campus
Hinxton
CB10 1SB
telephone: +44 1223 494552
fax: +44 1223 494512
email: tfreeman@hgmp.mrc.ac.uk
prestype: Platform
presenter: Tom Freeman
Tom Freeman, Richard Stephens, Debbie Williams, Jayn Wright, Rebecca Mayes,
Clare Potter, Laurie Scott, Yagnish Umrania*, Naran Hirani*, Tony Attwood*,
Jayne Vallance*, Alan Flook and Phil Gardner*
Microarray and *Informatics Development Groups, Human Genome Mapping Programme-Resource
Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SB.
The application of microarrays for gene expression profiling has been demonstrated to be one of the most powerful and direct ways of using the sequence data for functional studies. However, the true potential of this approach has yet to be realised widely, because previously access to the technology has been limited. The Microarray Programme at the HGMP-RC has a remit to develop and distribute the technology, specifically microarrays of human and mouse gene probes, to a UK academic user base.
We currently distribute microarrays of human and mouse genes, the probes having been generated by insert PCR from clone libraries. However, we are now increasingly turning to oligonucleotides as a probe resource for microarray fabrication. I will discuss our experience with this approach and other technological issues with respect to the manufacture and distribution of microarrays. This will be contrasted to our experience with the Affymetrix GeneChip system which we have been running for over a year. In addition, a considerable effort is currently being put in to developing the bioinformatics support for this programme and this will be discussed also. Finally, the future plans for the Microarray Programme at the HGMP-RC will be outlined.
http://www.hgmp.mrc.ac.uk/Research/Microarray/index.jsp
This work is funded by the MRC and BBSRC.