Our laboratory has developed strategies to bridge contig gaps occurring at human genomic regions which cannot be cloned and/or maintained faithfully in bacteria using the large cloning systems (PI/BAC/PAC,etc). Such strategies derive from the original HAEC technology which allows direct cloning of DNA in human cells as Human Artificial Episomal Chromosomes (Nature Genetics 8:33-41, 1994; Methods in Molecular Genetics 8:167-188, 1996).
We have now entered the phase of testing such technology for proof-of-concept. We will provide the human genome community with a resource to isolate and sequence refractory contig gaps. Hence, we are interested in hearing from the human genome community about persistent and refractory genomic gaps in order to identify potential candidates for the HAEC bridging technology. Suitable candidates are contig gaps which have been thoroughly tested on highly redundant large bacterial and yeast libraries and also shown to be missing in libraries prepared with different restriction enzymes.
Interested human genome laboratories should send their email reply to the attention of Jean-Michel Vos at the following address: angemari@gibbs.oit.unc.edu.
Dr. Jean-Michel H. Vos 349 Lineberger Comprehensive Cancer Center School of Medicine CB#7295 University of North Carolina at Chapel Hill Chapel Hill, NC 27599-7295 Fax: 919-966-3015 Vos lab web site: http://www.med.unc.edu/wrkunits/3ctrpgm/lccc/voslab
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