|Mapping chromosome 16. This much-reduced physical map of the short arm of human chromosome 16 summarizes the progress made at Los Alamos toward a complete map of the chromosome. A legible, fully detailed map of the chromosome is more than 15 feet long; only a few features of the map can be described here. Just below the schematic chromosome, the black arrowheads and the vertical lines extending the full length of the page signify "breakpoints" and indicate the portions of the chromosome maintained in separate cell cultures. The cultured portions typically extend from a breakpoint to one end of the chromosome. These breakpoints establish the framework for the Los Alamos mapping effort. Within this framework, some 700 megaYACs (shown in black) provide low-resolution coverage for essen-tially the entire chromosome. Smaller flow-sorted YACs (light blue, red, and black), together with about 4000 cosmids, assembled into about 500 cosmid contigs (blue and red), establish high-resolution coverage for 60% of the chromosome. Sequence-tagged sites (STSs) are shown as colored vertical lines above the megaYACs, and genes (green) and genetic markers (pink) that have been localized only to the breakpoint map are shown near the bottom. Also shown are cloned and uncloned disease regions, as well as those markers whose analogs have been identified among mouse chromosomes (see "The Mighty Mouse," pages 24Ð25).|
To Know Ourselveswas prepared at the request of the U.S. Department of Energy, Office of Health and Environmental Research, as an overview of the Human Genome Project.