Progress, and Applications
of the Human Genome Project
Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News Archive Edition
Human Genome News, January-March 1996; 7(5)
Researchers at the Human Genome Center of Lawrence Livermore National Laboratory have completed an integrated metric physical map of human chromosome 19 that spans over 95% of the euchromatin (about 50 Mb).
Appearing in the December 1995 issue of Nature Genetics (11, 422-27), the map is based on sets of overlapping cosmid clones (contigs); gaps between contigs were filled using various types of larger-insert clones. A "metric" scaffold was generated by using FISH in sperm pronuclei to estimate distances between selected cosmids. This FISH scaffold was integrated with other partial-order data generated by hybridization, STS screening, and restriction mapping using automated map assembly software.
The map, which is depicted in a special 4-page pull-out section, contains 51 "islands" of multiple clone types, with size, order, and relative distance known. Markers include more than 450 genes, polymorphic markers, STSs, and ESTs that are localized on average every 230 kb across the noncentromeric chromosome portion. Complete digest EcoR I cosmid restriction maps, also generated across 41 Mb, can be used to create subclone libraries for DNA sequencing. [More map data: http://bbrp.llnl.gov/bbrp/genome/genome.html]
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