Progress, and Applications
of the Human Genome Project
Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News Archive Edition
|Available in PDF
In this issue...
DOE '99 Oakland Highlights
In the News
Web, Other Resources, Publications
HGP Grantees Report Progress, Challenges
Report from 1999 DOE Genome Meeting
Some 360 researchers, program managers, and invited guests gathered in California on January 12-16 for the DOE Human Genome Program workshop. Plenary presentations and posters described a wide spectrum of accomplishments and activities, highlights of which are reported below.
A contingent of researchers from JGI reported on the challenges and triumphs involved in merging the sequencing capabilities of three national laboratories into a single, smoothly functioning unit while meeting much higher sequencing goals. Efforts were highly successful, and all objectives were reached as planned, with over 20 Mb of sequence submitted to GenBank in October 1998.
JGI converged the disparate sequencing processes of the three centers of Lawrence Berkeley, Lawrence Livermore, and Los Alamos national laboratories at the Production Sequencing Facility (PSF) in Walnut Creek, California. The team took up residence in one of the new buildings in December 1998, and Secretary of Energy Bill Richardson was keynote speaker at the April 19 formal PSF dedication (see box at right).
Clones containing target regions are isolated from 10x-coverage BAC libraries via a combination of colony hybridization and PCR approaches using STSs obtained mostly from public databases. Minimally overlapping clones that represent contiguous regions (contigs) of the chromosome are selected for sequencing. Contigs, expanded by end-sequence STS walking, are oriented by STSs developed from known genes and ordered genetic and RH markers. All clones are sized by pulsed-field gel electrophoresis, and their chromosome map locations are confirmed by FISH. Detailed information on STS and restriction maps is available on the Web site.
Quality Control and Assurance
"Data silos" in various locations currently are hard to connect, but eventually physical maps will become sequence maps as all marker and annotation data are merged. Querying, navigating, and displaying data soon will require seamless links to information on function, structure, and experimental results. Slezak emphasized the importance of implementing an infrastructure for functional genomics informatics.
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Last modified: Wednesday, October 29, 2003
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