In this issue...
HGP on Fast Track
Joint Genome Institute Exceeds Goal
Sequencing with BACs
with STCs and STSs
of BAC Clones and STC Data
Resource Success Story
Hunt SNPs for Variation, Disease
Sequencing the Human Genome?
Progress in Science
Promotes Remote Access to Instrumentation
Private-Sector Sequencing Project
In the News
Delivers C. elegans Sequence
Sequence Entire Genomes? Worm's Eye View
BER Research Update
1998 Human Genome Awards Announced
Consortium for Functional Genomics
Merges Offices, Web Sites
Microbial Genomes Searchable
Releases Chlorobium tepidum Sequence
MGP Abstracts Online
Ethical, Legal, and Social Issues
and Educational Resources
Lander, Genetics in the 21st Century
Rothstein, Genetic Privacy
Wilson, Gene Therapy Present & Future
Walters, Ethical Issues in Gene Therapy
Files on NPR, Internet
Course for Biology Teachers
at Proteins to Understand Expression
for Protein Analysis
100 Award Goes to LANL's SOLVE
Awards Proteomics Grant to Axys
coli Proteome Database
Genetics in Medicine
Organization for Rare Disorders
of Genetics to Medicine: New Website
Genetics Web Site
Website Offers Education in New Genetics
of Genetic Risks 2nd Edition
Research Genomics Online
Database Operations Restored
Silico Biology: Bioinformatics Journal
Methods Book Available
Programs at Sanger
Licenses Gene Logic Software
Database at LANL
at Jackson Laboratory
Database on Web
Web, Other Resources, Publications
Oakland Workshop Website
to Human Chromosomes
Office of Science Grants and Contracts
National Service Award Fellowships
Technologies for Molecular Analysis
Netork for Large-Scale Mouse Sequencing
Genomic Technology Development
Genome Research Funding
Meeting Calendars & Acronyms
and Biotechnology Meetings
Courses and Workshops
Mouse Resources Critical to Understanding Human Genome
Some 60 scientists met for 3 days in March 1998 in Bethesda, Maryland, to define priorities for producing resources to make the mouse a more valuable tool for understanding mammalian biology. Convened by NIH Director Harold Varmus, the Mouse Genomics and Genetics Resources Working Group's recommendations, as summarized by cochairs William Dove (University of Wisconsin) and David Cox (Stanford University), are outlined below. Total direct costs for the first year are estimated at $49.3 million.
The first follow-up meeting was held in October 1998 to discuss implementation of the March recommendations. Representatives from DOE and the U.K.'s Medical Research Council were present to develop a coordinated strategy and share expertise in this international effort.
Recommendations for structural analysis, functional analysis, and resources include the following:
- Generate an additional 60,000 new markers, identified as crucial for scientists who are cloning genes.
- Genotype inbred mouse strains and generate a low-resolution (5-cM) single-nucleotide polymorphism map to determine its value for mouse research.
- Sequence and map 3' ends of partial cDNAs and improve methods for isolating missing and full-length cDNAs.
- Generate 12 Mb of sequence for the first year and ramp up to 400 Mb within 5 years, obtaining a completed reference mouse genomic sequence by 2008.
- Develop standardized genome-wide mutagenesis protocols and improved tools and assays for characterizing phenotypes within new, specialized centers using the supermutagen ENU (ethyl nitrosourea, developed at Oak Ridge National Laboratory by William Russell).
- Develop phenotyping protocols in ENU centers and by individual investigators.
- Set up targeted mutagenesis programs to validate embryonic stem lines from different mouse strains for specialized uses.
- Couple molecular genotyping with the construction of congenic mouse strains.
- Develop cryopreservation methods and facilities for maintaining mutant mouse sperm and ovaries, thus reducing the cost of maintaining live animals.
- Build a new repository for live mouse strains.
- Evaluate and expand some existing databases.
- Train researchers in cryopreservation technology and animal pathology.
Note: More Mouse information can be found at:
The electronic form of the newsletter may be cited in the following style:
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Human Genome Program, U.S. Department of Energy, Human Genome News (v10n1-2).