73. A 12 Mbp Completely Contiguous Sequence-Ready BAC Contig in Human Chromosome 16p13.1-11.2 

Yicheng Cao, Hyung Lyun Kang, So Hee Dho¹, Diana Bocskai, Mei Wang, Xuequn Xu, Jun-Ryul Huh¹, Byeong-Jae Lee¹, Francis Kalush², Judith G. Tesmer³, Eunpyo Moon⁴, Norman A. Doggett³, Mark D. Adams², Melvin Simon, and Ung-Jin Kim 
¹ Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea; ² The Institute for Genomic Research, Rockville, Maryland; ³ Los Alamos National Laboratory, Los Alamos, New Mexico; and ⁴ Ajou University, Suwon, Korea 
simonm@cco.caltech.edu 

Here we present a 12 Mbp of BAC contiguity in the centromeric half of the chromosome 16p arm. The work initially involved extensive screening of deep human BAC libraries developed at Caltech using the STS markers that have been mapped to the target regions by Los Alamos National Laboratory. The positive BACs were characterized by sizing, FISH mapping, BAC end sequencing and sequence matches, and restriction fingerprint analysis. For the clones submitted for complete sequencing, genomic Southern blot analysis was performed to confirm the colinearity of the clones with the genomic DNA. 51 BAC clones in this region have been completely sequenced by TIGR. We post a comprehensive summary of the screening and characterization data for this and all other projects through our WEB site http://www.tree.caltech.edu. 

We use the AceDraw program developed by the CS140 team at Caltech for the construction and updating of the contig map. This tool allows freehand, real scale map drawing using various data and human judgement, and is capable of communicating with databases including ACeDB. We extensively utilize STS contents, restriction fingerprint data, and BAC end sequence matches for the establishment of clone-to-clone contiguity. For the gap closure, we also utilized BAC end probes, OVERGOes and additional BAC libraries such as RPCI.