|Genome Sequencing Technologies and Resources
DOE Human Genome Program Contractor-Grantee Workshop
Mouse cDNA Analysis by Automated Fluorescent 384 Capillary Sequencer System
(RISA; Riken Integrated Sequence Analysis System)
Hayashizaki Y, Okazaki Y, Kawai J, Carninci P, Shibata K, Ito
M, Tateno M, Sasaki N, Konno H, Sugahara Y, Kawahire S, Izawa M, Shibata
Y, Watahiki M, Yoneda Y, Tanaka T, Matuura S, Muramatsu, M
We have developed an automated fluorescent 384 capillary sequencer and a plasmid preparator that can extract a total of 40,000 plasmid clones in one day per a machine. A single automated sequencer can now process up to 2304 samples per day.
Full-length cDNA libraries based on the biotynlated cap trapper method have been developed and constructed. These full-length cDNA clones were picked into 384 well plates using Q-bot (Genetix inc.) and stored. We have sequenced more than 100,000 runs of 3'end sequences and classified them into more than 20,000 clusters. They are now open on the web (http://genome.rtc.riken.go.jp). We are now planning to sequence 1,000,000 runs in a coming year and categorizing the mouse full-length cDNAs.
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