141. Resources for Functional Genomics in Drosophila 

Gerald Rubin, Suzanna Lewis, Ling Hong, Damon Harvey, E. Jay Rehm, Amy Beaton, Peter Brokstein, Guochun Liao, Erwin Frise, and Allan Spradling 
University of California, Berkeley, California 
gerry@fruitfly.berkeley.edu 

A major goal of the Drosophila Genome Project is to biologically annotate the DNA sequence of the Drosophila melanogaster genome as it emerges and to provide community resources for functional genomics. To this end, we are carrying out several related projects: (1) insertional mutagenesis using P transposable elements that have been engineered to allow controlled misexpression, in addition to insertional inactivation, of the gene at the site of insertion; (2) generation of a "unigene" set of arrayed, full-length Drosophila cDNAs; (3) highly accurate DNA sequencing of a selected subset of these cDNAs; (4) single-pass sequencing of the remainder of the cDNAs as the corresponding genomic sequence becomes available to generate a transcript map of the genome; and (5) determination of the expression pattern of individual genes by tissue in situ hybridization to embryos at a variety of developmental stages and by hybridization to gene microarrays.