Genetic Mapping
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Complete the 2 to 5cM map by
1995.
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Develop technology for rapid
genotyping.
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Develop markers that are easier
to use.
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Develop new mapping
technologies.
Physical Mapping
-
Complete a sequence tagged site
(STS) map of the human genome at aresolution of 100 kb.
DNA Sequencing
-
Develop efficient approaches
to sequencing one to several megabase regions of DNA of high biological
interest.
-
Develop technology for high
throughput sequencing, focusing on systems integration of all steps from
template preparation to data analysis.
-
Build up a sequencing capacity
to allow sequencing at a collective rate of 50 Mb per year by the end of
the period. This rate should result in an aggregate of 80 Mb of DNA sequence
completed by the end of FY 1998.
Gene Identification
-
Develop efficient methods for
identifying genes and for placement of known genes on physical maps or
sequenced DNA.
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Technology
Development
-
Substantially expand support
of innovative technological developments as well as improvements in current
technology for DNA sequencing and for meeting the needs of the Human Genome
Project as a whole.
Model Organisms
-
Finish an STS map of the mouse
genome at a 300kb resolution.
-
Finish the sequence of the Escherichia
coli and Saccharomyces cerevisiae genomes by 1998 or
earlier.
-
Continue sequencing Caenorhabditis
elegans and Drosophila melanogaster genomes with the aim of
bringing C. elegans to near completion by 1998.
-
Sequence selected segments of
mouse DNA side by side with corresponding human DNA in areas of high biological
interest.
Informatics
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Continue to create, develop,
and operate databases and database tools for easy access to data, including
effective tools and standards for data exchange and links among
databases.
-
Consolidate, distribute, and
continue to develop effective software for largescale genome
projects.
-
Continue to develop tools for
comparing and interpreting genome information.
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Ethical, Legal, and Social Implications
-
Continue to identify and define
issues and develop policy options to address them.
-
Develop and disseminate policy
options regarding genetic testing services with potential widespread
use.
-
Foster greater acceptance of
human genetic variation.
-
Enhance and expand public and
professional education that is sensitive to sociocultural and psychological
issues.
Training
-
Continue to encourage training
of scientists in interdisciplinary sciences related to genome
research.
Technology Transfer
-
Encourage and enhance technology
transfer both into and out of centers of genome research.
Outreach
-
Cooperate with those who would
establish distribution centers for genome materials.
-
Share all information and materials
within 6 months of their development. This should be accomplished by submission
of information to public databases or repositories, or both, where
appropriate.
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