Sequencing Strategy. The directed sequencing strategy used
at LBNL involves four steps: (1) generate a P1-based physical map (using
STS-content mapping) to provide a set of minimally overlapping clones,
(2) shear and subclone each P1 clone into 3-kilobase fragments and identify
a minimally overlapping subclone set, (3) generate and map transposon inserts
in each subclone, and (4) sequence using commercial primer-binding sites
engineered into the transposon. Subclone sequences are then assembled and
edited, and the gaps are identified. P1 clones are reconstructed, and the
resulting composite data is analyzed, annotated, and finally submitted
to the databases. The production sequencing effort has generated 12 megabases
of finished, double-stranded genomic DNA sequence from both Drosophila
and human templates.
The 1997 DOE Human Genome Program Report is a two-part
report published in 1997 to reflect research and progress in the U.S. Department
of Energy Human Genome Program from 1994 through 1996, with specified updates
made just before publication. Part 1 is the program overview and
report on progress, and Part 2 consists of 1996 research abstracts.
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