Chromosome 16 Physical Map Part 2. (Part 1). A condensed chromosome 16 physical map constructed at Los Alamos National Laboratory (LANL) is shown in two parts. Besides facilitating the isolation and characterization of disease genes, the map provides the framework for a large-scale sequencing effort by LANL, The Institute for Genomic Research, and the Sanger Centre.
Distinct types of maps and data are shown as levels or tiers on the integrated map. At the top of each page is a view of the banded human chromosome to which the map is aligned. A somatic-cell hybrid breakpoint map, which divides the chromosome into 90 intervals, was used as a backbone for much of the map integration.
The physical map consists of both a low-resolution yeast artificial chromosome (YAC) contig map localized to and ordered within the breakpoint intervals with sequence tagged sites (STSs) and a high-resolution bacteria-based clone map. The YAC-STS map provides almost complete coverage of the chromosome's euchromatic arm, with STS markers on average every 100,000 bases.
A high-resolution, sequence-ready cosmid contig map is anchored to the YAC and breakpoint maps via STSs developed from cosmid contigs and by hybridizations between YACs and cosmids.
As part of the ongoing effort to incorporate all available loci onto a single map of this chromosome, the integrated map also features genes, expressed sequence tags, exons (gene-coding regions), and genetic markers.
The mouse chromosome segments at the bottom of
the map contain groups that correspond to human genes mapped to the regions
shown above them.
The 1997 DOE Human Genome Program Report is a two-part
report published in 1997 to reflect research and progress in the U.S. Department
of Energy Human Genome Program from 1994 through 1996, with specified updates
made just before publication. Part 1 is the program overview and
report on progress, and Part 2 consists of 1996 research abstracts.