Capillary Array Electrophoresis (CAE). CAE systems promise dramatically faster and higher-resolution fragment separation for DNA sequencing. A multiplexed CAE system designed by Edward Yeung (Iowa State University) has been developed for commercial production by Premier American Technologies Corporation (PATCO). In the PATCO ESY9600 model, DNA samples are introduced into the 96-capillary array; as the separated fragments pass through the capillaries, they are irradiated all at once with laser light. Fluorescence is measured by a charged coupled device that acts as a simultaneous multichannel detector. (Inset circle at upper left: Closeup view of individual capillary lanes with separated samples.) Because every fragment length exists in the sample, bases are identified in order according to the time required for them to reach the laser-detector region.
The 1997 DOE Human Genome Program Report is a two-part
report published in 1997 to reflect research and progress in the U.S. Department
of Energy Human Genome Program from 1994 through 1996, with specified updates
made just before publication. Part 1 is the program overview and
report on progress, and Part 2 consists of 1996 research abstracts.