Introduction to the Workshop
URLs Provided by Attendees

Abstracts

Mapping

Informatics

Sequencing

Instrumentation

Ethical, Legal, and Social Issues

Infrastructure

The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Accessing Genetic Information with DNA Arrays

Stephen P.A. Fodor and Robert Lipshutz
Affymetrix, 3380 Central Expressway, Santa Clara, CA 95051

Recent advances in DNA chip technology have produced a robust platform for accessing genetic information. This platform consists of DNA chips, instrumentation to read the chips and software to interpret the data. DNA oligonucleotide arrays are fabricated using high-resolution photolithography in combination with solid-phase oligonucleotide synthesis. Fluorescent nucleic acid targets are hybridized to the array. The hybridization pattern, as determined by epifluorescence microscopy, reveals details of the target sequence. Applications of this technology have been demonstrated including comparative gene sequencing, mutation detection, clone mapping and sequencing by hybridization. These applications are poised to address many of the goals of the Human Genome Project.