Introduction to the Workshop
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Abstracts

Mapping

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The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Integrated physical mapping of human cDNAs.

Mihael H. Polymeropoulos
National Center for Human Genome Research, NIH Bldg49 4A66, Bethesda Md 20892.

In the effort to characterize human cDNA derived partial sequence tags, we have developed STSs for 1,000 human cDNAs. With the use of human-rodent somatic cell hybrids, these cDNAs have been assigned to a chromosome. Although the information of the chromosome of origin is useful, it is the fine resolution mapping of the expressed sequences that maximizes their use human disease and related mapping efforts. In order to develop high resolution expression maps of the human genome, we have completed a pilot project for the high resolution mapping of chromosome 8 derived ESTs. In this project we screened the CEPH megabase YAC library for YAC addresses with each chromosome 8 STS. Two addresses were obtained for each cDNA and the database was searched for overlapping YACs at levels two and three. The original YAC addresses and the overlapping YACs were database searched for the presence of microsatellites with chromosome 8 origin. These searches were performed with the computer program yacsr. Of the chromosome 8 cDNAs mapped in this manner 60% were linked to a microsatellite on levels one or two. The relative locations were also verified by the use of chromosome 8 deletion hybrids. With the increasing density on YACs typed for genetic markers the success in fine mapping of the cDNAs will continue to increase. This mapping approach will provide powerful means of gene mapping by providing simultaneously a physical clone address and a location on the genetic map.