Introduction to the Workshop
URLs Provided by Attendees

Abstracts

Mapping

Informatics

Sequencing

Instrumentation

Ethical, Legal, and Social Issues

Infrastructure

The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Mapping Markers in Genomic Library Clones Using Oligonucleotide Arrays

R. Lipshutz, R.J. Sapolsky, S.P.A. Fodor
Affymetrix, Santa Clara, CA 95051

We have developed DNA chips and accompanying biochemical and informatic methods for detecting markers to order clones from genomic libraries. Through a series of enzymatic reactions, we selectively amplify and fluorescently label only those portions of the high molecular weight DNA which correspond to elements in a set of oligonucleotide markers. The complementary set of oligonucleotides to these markers is synthesized in a high-density array using light-directed combinatorial chemical synthesis. When the labeled target from a clone is hybridized to the spatially arranged probes, a pattern corresponding to the unique subset of markers contained in that clone is generated. The correlation of the hybridization patterns between the marker sets for two given inserts is an indication of their physical overlap. This methodology for screening and physically mapping clones into contigs is relatively fast and straightforward and can be easily adapted for automation.