Introduction to the Workshop
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The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.
Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.
Gene Isolation From Human Chromosome 19
Greg Lennon, Dominique Giorgi, Kimberly Lieuallen, Len Pennacchio, Christa Prange, and Sylvie Rouquier
Human Genome Center, L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore California 94550.
The goal of this effort is to isolate, sequence, and map coding regions located on human chromosome 19. First, 34 cDNAs selected by direct screening of cDNA libraries with ch 19 cosmids have been verified to correspond to ch 19 genes, and their corresponding genomic loci have been mapped. Second, hybrid selection experiments using flow-sorted ch 19 and arrayed cDNA libraries have resulted in cDNA sublibraries highly enriched for sequences from chromosome 19. A combination of both sequence and hybridization analysis of over 100 sublibrary clones indicates that over 50% have cognate sequences on chromosome 19. Some of the more than 60 new genes are being characterized in detail, including a candidate gene for two disorders linked to 19p, pseudoachondroplasia and multiple epiphyseal dysplasia.
The need for high-quality, arrayed cDNA libraries has also led us to form (in conjunction with C. Auffray) the Integrated Molecular Analysis of Gene Expression (IMAGE) Consortium. IMAGE is a collaborative group of over 27 laboratories working on the characterization of clones from shared arrayed cDNA libraries. Information derived from the study of specific clones is shared through the use of a collaborative database established and maintained (by M. Boguski and C. Tolstochev) at the NCBI. This database periodically transfers data directly to the public domain database dbEST. Most work to date has focused on the normalized infant brain cDNA library (from Dr. M. Soares, Columbia Univ.) as arrayed at LLNL, and then replicated and distributed worldwide. This array consists of 40,000 clones; over 10,000 single pass sequences have been generated, and over 1,000 cDNAs have been chromosomally mapped by Consortium members. We are currently arraying other high-quality normalized cDNA libraries, and invite the participation of laboratories willing to abide by the Consortium guidelines.
This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under contract no. W-7405-ENG-48.