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The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.
Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.
Generation and High Resolution Mapping of New STSs Derived From Chromosome 2 Microdissection Clones
Jeffrey C. Gingrich, Jeffrey A. Garnes, Jane E. Lamerdin and Lisa K.Scheidecker
Human Genome Center, L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94550.
In order to generate new resources for the mapping of human chromosome 2 we are generating new STSs from chromosome 2 microdissection clones. A series of microdissection clone libraries spanning ~20 Mb regions of chromosome 2 are being generated by Dr. F.-T. Kao and colleagues at the University of Colorado. From each library a number of unique sequence clones were identified and shown to be derived from chromosome 2. We have taken unique sequence clones from the 2Q1 and 2P1 libraries, libraries derived from the two telomeric regions of the chromosome, and have determined their DNA sequence. STS primer pairs were then determined using the Primer program. The STS primers were then tested against DNA from human, hamster and a monochromosomal hybrid cell line containing chromosome 2, GMl0826. To date, 35 new STSs have been generated, ~1 per Mb of the microdissected regions of the chromosome. Additional STSs will be generated from 8 other microdissection libraries from chromosome 2 as we obtain them. The STSs are being further localized on the YAC map of chromosome 2 by testing them against a set of regionally assigned YACs from the CEPH data set. The information obtained gives both an indication of the location of the STSs relative to other mapped markers on the chromosome and provides further validation of the CEPH data on chromosome 2.
This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under contract no. W-7405-ENG-48.