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Introduction to the Workshop
URLs Provided by Attendees

Abstracts

Mapping

Informatics

Sequencing

Instrumentation

Ethical, Legal, and Social Issues

Infrastructure

The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Efforts Toward the Development of a Transcription Map of Human Chromosome 16

The Los Alamos Genomics Group. Presented by Amanda Ford and Cleo Naranjo.
Life Sciences Division and Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, NM 87545.

Individual chromosomes provide the skeletal framework on which genomic data is organized. As the physical map and an ordered assemblage of molecular clones for chromosome 16 nears completion, we have embarked on the construction of an 'expressed sequence map' of the chromosome. To this end we have chosen the strategy of exon amplification to identify expressed sequences on chromosome 16. Our strategy employs 96-well plate pools of DNA from the flow sorted and arrayed chromosome 16 cosmid library as the substrate for exon trapping. At present we have generated 1800 exon clones from 75 of the 150 plates in the chromosome 16 library. We have sequenced over 500 of these clones and determined that approximately 60% appear unique. These sequences are being mapped to specific locations on chromosome 16 using a panel of somatic cell hybrids and molecular clones. Furthermore, these sequences are being subjected to database analysis to determine whether they represent previously characterized genes or contained conserved motifs that might provide some insight as to their biological function. Once completed, our map will serve two functions. First, it will allow us to pursue a detailed study of chromosome structure and gene organization. Lastly, it will provide investigators who have previously identified linkage to disease genes using chromosome 16 genetic reference markers, additional markers and candidate genes to analyze in their families.

Prepared by M.R. Altherr