Introduction to the Workshop
URLs Provided by Attendees
- Ethical, Legal, and Social Issues
The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.
Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.
Towards an STS content map of human chromosome 11: Localization of 910 YAC clones and contigs and assembly of a first generation physical map.
Glen A. Evans, Julie M. Bailis, Sylvia Thomas, Jason V. Khristich, Karin Diggle, Yelena Marchuck, Joshua Tobin, Stephen P. Clark, Annie Rodkins, Stewart Marcano, Allan C. Churukian, Jane S. Hutchinson, Yalin H. Wei, Ron Scott, Kim Jackson, Lori Romberg, Shane Probst, David Burbee, Michael W. Smith, Licia Selleri, John Quackenbush and Harold R. Garner.
McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75235-8591; Molecular Genetics Laboratory and Human Genome Center, The Salk Institute, La Jolla, California 92037; Amgen, Amgen Center, MS 239, Thousand Oaks, CA 92320; Biological Carcinogensis and Development Program, PRI/Dyncorp, Frederick Cancer Research and Development Center, National Cancer Institute, Frederick, MD 21702; Department of Pathology, Stanford University School of Medicine, Stanford CA 94305; Department of Genetics, Stanford University School of Medicine, Stanford CA 94305.
Physical mapping of human chromosomes at a resolution of the l00 kb to 1 mb will provide reagents for gene identification and templates for ultimately determining the complete DNA sequence. Sequence-tagged site (STS) content mapping coupled with large fragment cloning in yeast artificial chromosomes provides an efficient mechanism for producing first generation, low resolution maps of human chromosomes. Previously, we produced a set of standardized STSs for human chromosome 11 regionally localized by fluorescence in situ hybridization or somatic cell hybrid analysis. We used these, and other STSs to map over 900 YAC clones to chromosome 11, organized into 109 contigs. This data set spans 218 mb of coverage on the 126 mb chromosome, includes a large number of (CA)n repeats and other genetically defined markers, and represents a first order approximation of a physical map of human chromosome 11. This set of clones, contigs, genetic markers and associated STSs will provide the material for the production of a continuous overlapping set of YACs as well for high resolution physical mapping based upon sampled sequencing, and ultra-rapid genotyping using DNA chips.
This work was supported by the DOE Genome Program, the National Center for Human Genome Research, and the G. Harold and Leila Y. Mathers Charitable Foundation. MWS was a Human Genome Distinguished postdoctoral fellow of the DOE and JQ is a Special Research Career Emphasis Awardee of NCHGR.