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Introduction to the Workshop
URLs Provided by Attendees

Abstracts

Mapping

Informatics

Sequencing

Instrumentation

Ethical, Legal, and Social Issues

Infrastructure

The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Fluorescent in situ Hybridization Mapping of cDNA Clones from an Unusual Human Library

Karen Denison, Joe M. Gatewood
Genomics and Structural Biology; LS-2, MS M880; Los Alamos National Laboratory; Los Alamos, New Mexico 87545

Hydatidiform mole results from an exclusively paternal fertilization event and is characterized by unusual trophoblastic growth without an apparent fetus. Incidence is approximately 1/1000 pregnancies. Tissue from a 14-week pregnancy was used for the production of size selected, directionally cloned cDNA libraries. Over thirty selected clones from one library were mapped using fluorescent in situ hybridization (FISH) to human R-banded metaphase chromosomes after the method of Korenberg, et al.[1]. Six of these clones represent known genes as described in GenBank. Five of these previously unmapped genes (M-type pyruvate kinase[2], ribosomal protein S3[3], human growth hormone hGH[4], rho GDP-dissociation inhibitor 2[5], and GATA-binding protein 2[6]) have been assigned and the assignment of the sixth gene (type IV collagenase[7]) has been refined.

[1] Korenberg, J K, Chen, X.N., Doege, K, Grover, J. and Roughley, P.J. (1993). Assignment of the human aggrecan gene (AGCl) to 15q26 using fluorescent in situ hybridization analysis. Genomics 16 546-548.
[2] GenBank accession X56494.
[3] GenBank accession X55715.
[4] GenBank accession J00148, K00612.
[5] GenBank accession X69549.
[6] GenBank accession M68891.
[7] GenBank accession J03210, J03070, J05471.