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The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Human Chromosome 19: A Fluorescence in situ Hybridization Map with Genomic Distance Estimates for 196 Sequential Intervals Separating 198 Ordered Cosmid Reference Points and Spanning 50 Mb

Brigitte F. Brandriff, Laurie A. Gordon, Anne S. Olsen, Anthony V. Carrano, Anne Bergmann, Mari Christensen, Anne Fertitta, Linda Danganan, Denise Lee, Linda K. Ashworth, David O. Nelson, and Harvey W. Mohrenweiser
Human Genome Center, L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore California 94550.

A physical map of human chromosome 19 has been constructed by fluorescence in situ hybridization of a set of reference cosmids to metaphase chromosomes, somatic interphases, and sperm pronuclear interphases. Genomic distance estimates were derived by reference to a standard curve relating known genomic distances and physical distances measured in pronuclei [1]. The map spans 50 Mb and was generated with multiple, partially overlapping estimates of genomic distances for 196 intervals separating 198 sequentially ordered cosmid reference points. The average distance separating pairs of cosmids in the p arm was 250 kb, with a range from 50 to 700 kb; in the q arm, 270 kb with a range of 50 to 840 kb. The mapped elements included cosmids positive for 33 genes or gene family members and 5 polymorphic markers in the p arm; and in the q arm, cosmids positive for 50 genes or gene family members, and 26 polymorphic markers. Overall, there were 22 cosmids from 19p13.3; 19 cosmids from p13.2; 21 cosmids from p13.1; 18 cosmids from p12; 20 cosmids from q12; 27 from q13.1; 23 from q13.2; 23 from q13.3; and 25 from q13.4. An analysis of replicate genomic distance estimates generated for a subset of 45 cosmid pairs showed that over 98% of the variation between measurements was due to properties inherent in the decondensed pronuclear chromatin with less than 2% due to incidental experimental factors. Results of a comparison between a subset of genomic distance estimates generated by FISH in pronuclei and genomic distance estimates for the same intervals obtained by restriction mapping indicated close agreement between the two sets of data in most cases. The FISH map is being utilized as a metric backbone for other chromosome 19 mapping efforts (see related LLNL posters).

This work was performed under the auspices of The U.S. Department of Energy by Lawrence Livermore National Laboratory under contract no.W-7405-ENG-48.

[1] Brandriff et al. (1994), Genomics, in press.