Introduction to the Workshop
URLs Provided by Attendees
- Ethical, Legal, and Social Issues
The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.
Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.
MAPPING SEQUENCE TAGGED SITES TO DISTINCT SEGMENTS OF CHROMOSOME 16
The Los Alamos Genomics Group and D.F. Callen. Presented by M.R Altherr.
Life Sciences Division and Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, NM, USA; Department of Cytogenetics and Molecular Genetics, Women's and Children's Hospital, Adelaide, SA, Australia.
We have used a panel of 70 human::murine somatic cell hybrids containing discrete segments of human chromosome 16 to physically localize 310 sequence tagged sites (STSs) derived from an extensive collection of fingerprinted chromosome 16 specific cosmids. The distribution of these sequences along the chromosome appears to be random. Fewer than 10% of the sequences failed to unambiguously identify chromosome 16 locations. Along with the 229 PCR based probes currently listed in GDB this now represents an average spacing of one STS for every 176 kbp of chromosome 16. Our localized STSs provide physical anchors for both cosmid and YAC contigs. Additional STSs will be generated as a result of our chromosome 16 expressed sequence studies using clones resulting from exon amplification. As a consequence, any additional STSs added to the map by us will also represent potential coding sequences. We anticipate that these sequences will provide the starting point for a chromosome 16 transcription map.