Introduction to the Workshop
URLs Provided by Attendees

Abstracts

Mapping

Informatics

Sequencing

Instrumentation

Ethical, Legal, and Social Issues

Infrastructure

The electronic form of this document may be cited in the following style:
Human Genome Program, U.S. Department of Energy, DOE Human Genome Program Contractor-Grantee Workshop IV, 1994.

Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop. Inaccuracies have not been corrected.

Molecular Biology Laboratories on Microchips

Stephen C. Jacobson, Alvin W. Moore, Jr., and J. Michael Ramsey
Chemical & Analytical Sciences Division, Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831-6142

We have recently demonstrated the ability to perform a number of chemical analysis manipulations on microfabricated devices that have areas on the order of 1 cm(2) and volumes of a few tenths of a cubic centimeter. These devices are fabricated using conventional micromachining techniques on glass and fused silica substrates. Microchannels and microreactors are fabricated by chemically etching features in a planar substrate. The channels and reaction chambers are closed by bonding a cover plate over the machined areas. Microfluidic manipulation is effected through the use of electroosmotic flow. We have demonstrated the ability to manipulate fluid volumes as small as 100 picoliters with the precision of less than 1% rsd. Several chemical separation techniques have been demonstrated in our laboratory on microfabricated devices including free solution electrophoresis, open tubular electrochromatography, micellar electrokinetic capillary chromatography, and capillary gel electrophoresis. Moreover, the ability to perform chemical reactions "on chip" under computer control with picoliter and nanoliter volumes has been demonstrated. Performance of all these separation techniques have been either equivalent to or greater than conventional laboratory devices. We are now applying these new capabilities to molecular biology problems.

Research sponsored by U.S. Department of Energy, Office of Basic Energy Sciences, under contract DE-AC05-84OR21400 with Martin Marietta Energy Systems, Inc.