|Function and cDNA Resources Section
DOE Human Genome Program Contractor-Grantee
99. The RIKEN Mouse Full-Length cDNA Encyclopedia
Piero Carninci, Kazuhiro Shibata, Masayoshi Itoh, Hideaki Konno, Jun Kawai, Yuko Shibata, Yuichi Sugahara, T. Endo, Y. Ozawa, Yoshifumi Fukunishi, Atsushi Yoshiki, M. Kisakabe, Masami Muramatsu, Yasushi Okazaki, and Yoshihide Hayashizaki
Genome Science Laboratory, RIKEN, Tsukuba Life Science Center, 3-1-1 Koyadai, Japan
We report the ongoing efforts to prepare the mouse full-length cDNA Encyclopedia. We have, to the date of the submission of this abstract, constructed more than 150 full-length cDNA libraries. Libraries were prepared with the CAP trapper full-length cDNA selection method coupled to the trehalose-thermoactivated reverse transcriptase, in order to clone long full-length cDNAs. Additionally, in most libraries, cDNAs were normalized and subtracted to isolate rarely expressed full-length cDNAs. To date, we have clustered 100,907 3' end sequences containing at least half of all mouse full-length cDNAs in 690,130 successful sequencing reactions from 5'-end validated libraries. We have also established technology and mRNA resources to collect the majority of remaining mouse expressed sequences.
We will discuss about library preparation and tissue selection strategy, quality of cDNA libraries in terms of complexity and full-length cDNAs presence and coverage of mouse genes by our clones.
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