DOE Human Genome Program Contractor-Grantee Workshop VIII
February 27-March 2, 2000  Santa Fe, NM

77. PUMA2 -- An Environment for Comparative Analysis of Metabolic Subsystems and Automated Reconstruction of Metabolism of Microbial Consortia and Individual Organisms from Sequence Data

Natalia Maltsev and Mark D'Souza

Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, IL

maltsev@mcs.anl.gov

We have developed a working prototype of interactive environment PUMA2 which intends to accomplish the following goals:

1. Allow comparative analysis of the metabolic subsystems in different organisms

2. Provide a framework for the automated reconstruction of the metabolism of microbial consortia and individual species

3. Provide a framework for representation of the expression data.

Analysis of data in PUMA2 is based on an original approach for representation of metabolism as a network of interconnected modules connected to sequence data. The results of such analyses will be presented in graphical form based on hierarchical representation of the functional subsystems and annotated with sequence data and literature information.