DOE Human Genome Program Contractor-Grantee
48. Annotation and Analysis of the Draft Sequence of 16Q12
Jung-Rung Wu, Mark O. Mundt, Cliff S. Han, Kristina Kommander, Robert D. Sutherland, Lela Tatum, Norman A. Doggett, and Larry L. Deaven
Bioscience Division and DOE Joint Genome Institute, Los Alamos National Laboratory, Los Alamos, NM 87545
We have completed a map and chosen a tiling set of more than 40 spanning BACs, most often from the RPCI-11 library, for a region that covers more than 5 Mb of human 16q12, encompasing a locus for inflamatory bowel disease (IBD1). These BACs have been selected by a combination of overgo hybridization, restriction map assembly by both fingerprinting and sequence prediction, and BAC end sequence searches. Sequencing has been completed to levels ranging from light shotgun to Bermuda finished bases. We will present annotation and statistical results of our analysis of the draft sequence we have achieved thus far and show how these results compare with ~ 5 Mb of sequence from 16p13.3.
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