DOE Human Genome Program Contractor-Grantee Workshop VIII
February 27-March 2, 2000  Santa Fe, NM

20. New Technologies for Genome Sequencing and Expression Analysis

Wayne P. Rindone, John Aach, Martha Bulyk, George M. Church, Jason Hughes, Abby Mcguire, Pam Ralston, Martin Steffen, and Saeed Tavazoie

Department of Genetics, Harvard Medical School, Boston, MA 02115

wrindone@arep.med.harvard.edu

We pursue new, basic technologies for collecting low-cost, quantitative molecular data, useful for a variety of sequencing and/or functional genomics goals. We have developed a set of new methods for producing microarrays of Acrydite-immobilized PCR colonies and subsequent Fluorescent In Situ Sequencing (FISSEQ) on the slides. We have integrated mRNA expression data from SAGE, Chip, and microarray experiments into the ExpressDB database and developed a flexible Web-based search engine for exploring the data for clustering and DNA-motif analysis. The process also lead to insights into methodological improvements that would make such information more amenable to comparison among other laboratories.

Nucleic Acids Res 27(24):e34;pp. 1-6

Nature Genetics 22:281-5

http://arep.med.harvard.edu/

(LBNL) and DE-AC05-96OR22464 (ORNL).