|Microbial Genome Program Section
DOE Human Genome Program Contractor-Grantee
119. The Caulobacter crescentus Genome Sequencing Project
Tamara Feldblyum1, William C. Nierman1, Nikhil Phadke2, Peter Ulintz2, and Janine Maddox2
1The Institute for Genomic Research, Rockville, MD and 2Department of Biology, University of Michigan
Caulobacter crescentus is a member of the alpha subclass of the proteobacteria which also include Rickettsia, Rhizobium, Agrobacterium and Brucella species. It is the most prevalent non-pathogenic bacterium in nutrient-poor fresh water streams and is also found in marine environments. It is one of the organisms responsible for sewage treatment. Caulobacters are being modified for use as a bioremediation agent for removing heavy metals from wastewater.
Caulobacter crescentus has been extensively studied because it exhibits a well-defined developmental pattern that is independent of environmental stress. The free-swimming morphologically distinct swarmer cell progresses to an anchored stalked cell, the only cell type capable of genome replication and cell division. Cell division of the stalked cell splits out a swarmer daughter cell.
C. crescentus has a genome size of 4 Mb, with G+C content of about 66.5%. Tremendous power for genome assembly was brought to this project through the use of a 2 and 10 kb insert size 2 plasmid library strategy. In sequencing this organism at TIGR, 65,588 random sequence reads from both ends of plasmid clones were used to assemble the genome into only five groups comprising essentially all of the genome sequence. A preliminary review of C. crescentus ORFs revealed by the sequence is provided.
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