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New Phytologist manuscript NPH-RAMP-2007-06523
Supplemental Figure 1. Distribution of microsatellites including the SSR primers along each Populus chromosome. Sequences are arranged every 1 Mb from one end of the chromosome to the other. The physical length is estimated as the mapped sequence scaffold lengths plus the averaged gap length across scaffolds. The A, T, G and C sequences are represented with the black regions of the vertical bar, whereas N readings and gaps are drawn in gray. We used four colors to indicate the different classes of microsatellite repeats. The blue, red, yellow and green horizontal bars in the chart corresponded to penta-, tetra-, tri- and dinucleotide repeats, respectively. To the left of the distribution is a size ruler in base pairs (available at: http://www.ornl.gov/sci/ipgc/new_phytologist_supplemental.htm).
Supplemental Table 1. SSR primer sequences and parameters database developed from the mapped sequence scaffolds on each Populus chromosome. The primers are in 19 EXCEL spreadsheets that correspond to the 19 chromosomes, respectively (available at: href="http://www.ornl.gov/sci/ipgc/new_phytologist_supplemental.htm). Note: “LP” is the abbreviation of “left primer” and “RP” represents “right primer”. Tm is the melting temperature of the oligonucleotide primer.
- Supplemental Table 1 LG-1
- Supplemental Table 1 LG-2
- Supplemental Table 1 LG-3
- Supplemental Table 1 LG-4
- Supplemental Table 1 LG-5
- Supplemental Table 1 LG-6
- Supplemental Table 1 LG-7
- Supplemental Table 1 LG-8
- Supplemental Table 1 LG-9
- Supplemental Table 1 LG-10
- Supplemental Table 1 LG-11
- Supplemental Table 1 LG-12
- Supplemental Table 1 LG-13
- Supplemental Table 1 LG-14
- Supplemental Table 1 LG-15
- Supplemental Table 1 LG-16
- Supplemental Table 1 LG-17
- Supplemental Table 1 LG-18
- Supplemental Table 1 LG-19
Supplemental Table 2.The distribution test of SSR densities in a 2-Mb sliding window along each Populus chromosome. The valid reading lengths represent the sum of A, T, G and C bases in each window. The density in each window was verified a goodness-of-fit to a normal distribution by the Anderson-Darling test for normality. Confidence intervals were calculated based on mean ± 1.96 s.d. and mean ± 2.58 s.d., with corresponding p<0.05 and p<0.01 significance levels, respectively. **+, significantly overabundant at p<0.01; *+, significantly overabundant at p<0.05; **-, significantly under abundant at p<0.01; *- significantly under abundant at p<0.05. Data available at: http://www.ornl.gov/sci/ipgc/new_phytologist_supplemental.htm.
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