Dale Pelletier

Dale A. Pelletier

Biological and Nanoscale Systems
BioSciences Division
Oak Ridge National Laboratory
Oak Ridge, TN 37831-6341
Phone: (865) 576-2857
pelletierda@ornl.gov

Dale Pelletier is a staff scientist in the Bioscience Division at Oak Ridge National Laboratory. He received his B.S. in Biochemistry from the University of Maine in Orono, ME in 1986, his Ph.D. in Microbiology from the University of Iowa in 2000 and post-doctoral work at Stanford University in the Civil and Environmental Engineering Department on the topic of anaerobic aromatic degradation. His current research focuses on molecular analysis of plant-microbe and microbe-microbe interactions. My group applies isolation and characterization of microbes from the rhizosphere and root tissues of Populus trees with the goal of understanding mechanisms of microbial interactions and influence on host plant.

Publications

  1. Heidi A. Crosby, Dale A. Pelletier, Gregory B. Hurst, and Jorge C. Escalante-Semerena. (2012) "System-wide Studies of N-Lysine acetylation in Rhodopseudomonas palustris reveals substrate specificity of protein Acetyltransferases. JBC.  287(19):15590-15601.
  2. David J. Weston, Dale A. Pelletier, Jennifer L. Morrell-Falvey, Timothy J. Tschaplinski, Sara S. Jawdy, Tse-Yuan Lu, Sarah J. Melton, Madhavi Martin, Christopher W. Schadt, Abhijit A. Karve, Jin-Gui Chen, Mitchel J. Doktycz, Gerald A. Tuskan. (2012) Pseudomonas fluorescens induces strain-dependent and strain-independent host plant responses in defense networks, primary metabolism, photosynthesis and fitness. MPMI. 25(6):765-778.
  3. Brown, Steve, Dawn Klingeman, Tse-Yuan Lu, Courtney Johnson, Sagar Utturkar, Miriam Land, Christopher Schadt, Mitchel Doktycz, and Dale Pelletier (2012) Draft Genome Sequence of Rhizobium sp. PDO1-076, a bacterium isolated from Populus deltoides. JBact. 194(9):2383-2384.
  4. Suresh AK, Pelletier DA, Wang W, Morrell-Falvey J, Doktycz M. “Cytotoxicity Induced by Engineered Silver Nanocrystallites is Dependent on Surface Coatings and Cell Types” Langmuir. 2012 Feb 7;28(5):2727-35
  5. Neil Gottel, Hector Castro, Marilyn Kerley, Zamin Yang, Dale Pelletier, Mircea Podar, Tatiana Karpinets, Edward Uberbacher, Gerald Tuskan, Rytas Vilgalys, Mitchel Doktycz, and Christopher Schadt. (2011)  Populus deltoides roots harbor distinct microbial communities within the endosphere and rhizosphere across contrasting soil types.  AEM 77(17):5934-44
  6. Pelletier, Dale A., Anil K. Suresh, Gregory A. Holton, Catherine K. McKeown, Wei Wang, Baohua Gu, Ninell P. Mortensen, David P. Allison, David C. Joy, Martin R. Allison, Steven D. Brown, Tommy J. Phelps, Mitchel J. Doktycz.  (2010) Engineered cerium oxide nanoparticles: Effects on bacterial growth and viability. AEM 76(24):7981-89.
  7. Suresh, Anil K., Dale A. Pelletier, Wei Wang, Ji-Won Moon, Baohua Gu, Ninell P. Mortensen, David P. Allison, David C. Joy, Tommy J. Phelps, Mitchel J. Doktycz. (2010) Silver nanocrystallites: Biofabrication using Shewanella oneidensis, and an evaluation of their comparative toxicity on Gram-negative and Gram-positive bacteria. ES&T 44(13):5210-5215.
  8. Edwards AN, Fowlkes JD, Owens ET, Standaert RF, Pelletier DA, Hurst GB, Doktycz MJ, Morrell-Falvey JL. (2009) An in vivo imaging-based assay for detecting protein interactions over a wide range of binding affinities. Anal Biochem. 395(2):166-77.
  9. Yang S., K.M. Pappas, L.J. Hause, M.L. Land, G-L. Chen, G.B. Hurst, C. Pan, V. Kouvelis, M. Typas, D.A. Pelletier, D.M. Klingeman, Y-J. Chang, N.F. Samatova, and S.D. Brown. (2009) Reannotation of the Zymomonas mobilis ZM4 genome. Nature Biotechnology 10:893-4.
  10. Kalluri, U. P.K. Lankford, Ranjan P, G.B Hurst, D.A. Pelletier. Shotgun profiling of Populus developing xylem proteome. Proteomics 9(21):4871-4880.
  11. W. Judson Hervey, IV, Gurusahai Khalsa-Moyers, Patricia K. Lankford, Elizabeth T. Owens,Catherine K. McKeown, Tse-Yuan Lu, Linda J. Foote, Keiji G. Asano, Jennifer L. Morrell-Falvey, W. Hayes McDonald, Dale A. Pelletier, and Gregory B. Hurst. (2009) Evaluation of affinity-tagged protein expression strategies using local and global isotope ratio measurements. J. Proteome Res. 8(7):3675–3688.
  12. Joshi, GS, Romagnoli S, VerBerkmoes NC, Hettich RL, Pelletier DA, Tabita FR. (2009) Differential synthesis of form I RubisCO in Rhodopseudomonas palustris CGA010 under photoheterotrophic growth conditions with reduced carbon sources. J. Bacteriol. 191(13):4243-4250.
  13. Karpinets TV, Pelletier DA, Pan C, Uberbacher EC, Melnichenko GV, Hettich RL, Samatova NF. Phenotype fingerprinting suggests the involvement of single-genotype consortia in degradation of aromatic compounds by Rhodopseudomonas palustris. PLoS One. 2009;4(2):e4615.
  14. Oda Y, Larimer FW, Chain PSG, Malfatti S, Shin MV, Vergez LM, Hauser L, Land ML, Braatsch S, Beatty JT, Pelletier DA, Schaefer AL, Harwood CS. (2008). Multiple genome sequences reveal adaptations of a phototrophic bacterium to sediment microenvironments. Proc Natl Acad Sci USA 105(47):18543-18548.
  15. Pelletier, D.A., G.B. Hurst, L.J. Foote, P.K. Lankford, C.K. McKeown, T-Y. Lu, D.D. Schmoyer, M.B. Shah, W.J. Hervey IV, W.H. McDonald, B.S. Hooker, W.R. Cannon, D.S. Daly, J.M. Gilmore, H.S. Wiley, D.L. Auberry, Y.Wang, F.W. Larimer, S.J. Kennel, M.J. Doktycz, J.L. Morrell-Falvey, E.T. Owens, M.V. Buchanan. A general system for studying protein-protein interactions in Gram-negative bacteria. J. Proteome Res., 7 (8), 3319–3328, 2008.
  16. Parales, R.E., J.V. Parales, D.A. Pelletier, and J.L. Ditty. (Eds.) A.I. Laskin, S. Sariaslani and G.M. Gadd. Chapter 1 Diversity of microbial toluene degradation pathways. Adv.Appl.Microbiol., Academic Press: 2008; 64:1-73
  17. Chongle, P., Y. Oda, P. K. Lankford, B. Zhang, N. F. Samatova, D. A. Pelletier, C. S. Harwood, and R. L. Hettich. Characterization of anaerobic catabolism of p-coumarate in Rhodopseudomonas palustris by integrating transcriptomics and quantitative proteomics. Mol.Cell.Proteomics, 2008; 7:938-948.
  18. Sharp, J. L.; Anderson, K. K.; Hurst, G. B.; Daly, D. S.; Pelletier, D. A.; Cannon, W. R.; Auberry, D. L.; Schmoyer, D. D.; Hayes McDonald, W.; White, A. M.; Hooker, B. S.; Victry, K. D.; Buchanan, M. V.; Kery, V.; Wiley, H. S. Statistically Inferring Protein-Protein Associations with Affinity Isolation LC-MS/MS Assays. J. Proteome Res.; 2007; 6(9); 3788-3795.
  19. Pan C, Kora G, McDonald WH, Tabb DL, VerBerkmoes NC, Hurst GB, Pelletier DA, Samatova NF, Hettich RL. ProRata: A quantitative proteomics program for accurate protein abundance ratio estimation with confidence interval evaluation. Anal Chem. 2006 Oct 15;78(20):7121-31.
  20. Pan C, Kora G, Tabb DL, Pelletier DA, McDonald WH, Hurst GB, Hettich RL, Samatova NF. Robust estimation of peptide abundance ratios and rigorous scoring of their variability and bias in quantitative shotgun proteomics. Anal Chem. 2006 Oct 15;78(20):7110-20.
  21. Connelly HM, Pelletier DA, Lu TY, Lankford PK, Hettich RL. Characterization of pII family (GlnK1, GlnK2, and GlnB) protein uridylylation in response to nitrogen availability for Rhodopseudomonas palustris. Anal Biochem. 2006 Oct 1;357(1):93-104.
  22. VerBerkmoes, N.C., M. B. Shah, P. K. Lankford, D. A. Pelletier M. B. Strader, D. L. Tabb, W. H. McDonald, J. W. Barton, G. B. Hurst, L. Hauser, B. H. Davison, J. T. Beatty, C. S. Harwood, F. R. Tabita, R. L. Hettich, and F. W. Larimer. 2006. Determination and Comparison of the Baseline Proteomes of the Versatile Microbe Rhodopseudomonas palustris under its Major Metabolic States. J. Proteome Res., 5(2):287 -298.
  23. Thormann, K.M., R.M. Saville, S. Shukla, D.A. Pelletier, and A.M. Spormann. 2004. Initial Phases of Biofilm Formation in Shewanella oneidensis MR-1. J. Bacteriol. 186(23):8096–8104.
  24. Strader, M.B., N.C. VerBerkmoes, D.L. Tabb, H.M. Connelly, J.W. Barton, B.D. Bruce, D.A. Pelletier, B.H. Davison, R.L. Hettich, F.W. Larimer, and G.B. Hurst. 2004. Characterization of the 70S Ribosome from Rhodopseudomonas palustris using an Integrated “Top-Down” and “Bottom-Up” Mass Spectrometric Approach. J Proteome Res. 3(5):965-978.
  25. Larimer, F.W., P. Chain, L. Hauser, J. Lamerdin, S. Malfatti, L. Do, M. Land, D.A. Pelletier, J.T. Beatty, A.S. Lang, F.R. Tabita, J.L. Gibson, T.E. Hanson, C. Bobst, J.L. Torres Y Torres, C. Peres, F.H. Harrison, J. Gibson and C.S. Harwood. 2004. Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris. Nature Biotechnology, 22(1):55-61.
  26. Larimer, F.W., P. Chain, L. Hauser, J. Lamerdin, S. Malfatti, L. Do, M. Land, D.A. Pelletier, J.T. Beatty, A.S. Lang, F.R. Tabita, J.L. Gibson, T.E. Hanson, C. Bobst, J.L. Torres Y Torres, C. Peres, F.H. Harrison, J. Gibson and C.S. Harwood. 2004. Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris. Nature Biotechnology, 22(1):55-61.
  27. Doktycz, M.J., C. Sullivan, P. R. Hoyt, D. A. Pelletier, S. Wu and D. P. Allison. 2003. AFM imaging of bacteria in liquid media by immobilization on gelatin coated mica surfaces. Ultramicroscopy, 97:209-216.
  28. Johnson, H.A., D.A. Pelletier and A.M. Spormann. 2001. Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme. J. Bacteriol. 183:4536-42.
  29. Pelletier, D.A. and C.S. Harwood. 1999. 2-Hydroxycyclohexanecarboxyl Coenzyme A dehydrogenase, an enzyme characteristic of the anaerobic benzoate degradation pathway used by Rhodopseudomonas palustris. J. Bacteriol. 182:2753-2760.
  30. Gibson, K.J., D.A. Pelletier, and I.M. Turner, Sr. 1999. Transfer of sulfur to biotin from biotin synthase (BioB protein). Biochem. Biophys. Res. Comm. 254:632-635.
  31. Pelletier, D.A. and C.S. Harwood. 1998. 2-Ketocyclohexanecarboxyl coenzyme A hydrolase, the cleavage enzyme required for anaerobic benzoate degradation by R. palustris. J. Bacteriol. 180:2330-2336.
  32. Egland, P.E., D.A. Pelletier, M. Dispensa, J. Gibson and C.S. Harwood. 1997. A cluster of bacterial genes for anaerobic benzene ring reduction. Proc.Nat.Acad.Sci.USA. 94:6484-6489.
  33. Haddock, J.D., D.A. Pelletier and D.T. Gibson. 1997. Purification and properties of ferredoxinBPH, An component of biphenyl 2,3-dioxygenase of Pseudomonas sp. strain LB400. Journal of Industrial Microbiology and Biotechnology 19:355-359.
  34. Paster, B.J., D.A. Pelletier, F.E. Dewhirst, W.G. Weisburg, V. Fussing, L.K. Poulsen, S. Dannenberg and I. Schroeder. 1996. Phylogenetic position of the spirochetal genus Cristispira. Appl. Environ. Microbiol. 62:942-946.
  35. An, Q., G. Radcliffe, R. Vassallo, D. Buxton, W. J. O’Brien, D.A. Pelletier, W. G. Weisburg, J. D. Klinger, and D. M. Olive. 1992. Infection with a plasmid-free variant chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection. J. Clin. Microbiol. 30:2814-2821.
  36. Weisburg, W.G., S.M. Barns, D.A. Pelletier, and D.J. Lane. 1991. 16S Ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697-703.