Poster Presentation 2-20

Gibberelic Acid Production Optimization

César A. Jiménez-Sierra, Fabián Robles-Martínez, J. Manuel Muñoz-Aguilar

and Enrique Durán-Páramo^*

Bioprocess Department, UPIBI

National Polytechnic Institute,

Ave. Acueducto s/n

Ticomán 07340 México, D.F.

Phone: (52).55.57.29.60.00 ext. 56347

Fax: (52).55.57.29.60.00 ext. 56305

E-mail: eduran@acei.upibi.ipn.mx

Gibberellic acid production has been widely studied with different strains of Gibberella fujikuroi in different culture media. At present, the production of gibberellic acid by submerged fermentation with the filamentous fungi Gibberella fujikuroi can be a practical alternative to increase the production of vegetal biomass for the production of more and better foods, fuels and chemicals.

Gibberellic acid represents one the most important plant growth regulators. In this work, we present the preliminary results on optimization of the gibberellic acid production cells of Gibberella fujikuroi NRRL-2278. We use a complex culture medium and try to optimize the culture conditions in batch reactors by using a method of surface responses for the production of the plant regulator. Using this statistic method we try to select the most suitable culture medium and fermentation conditions for the production of gibberellic acid using this particular strain.