Poster Presentation 1B-57

Adaptability of Lentinula edodes Strains to Enzyme Production

in Sawdust and Waste of Eucalypt

¹Ezequiel M. Silva and ¹Adriane M. F. Milagres*

¹Depto. Biotecnologia – FAENQUIL

CP 116, CEP 12600-000

Lorena, SP, Brazil

Phone: 55-12-3159-5000

Fax: 55-12-3153-3165

E-mail: adriane@debiq.faenquil.br

Lentinula edodes utilize a variety of lignocellulosic residues, convert them into human food, and produce extracellular enzymes that can be used in several industrial processes. In this work we tested the ability of nine strains of L. edodes to grow in eucalypt residue in comparison with the traditional eucalypt sawdust. The eucalypt residue is composed of ground barks, branches and leaves left in the forest. Both substrates were supplemented with rice bran (80:20 w/w) at 80% humidity and 10 g of mixture were inoculated with three discs (Æ= 5 mm) of agar mycelium. After thirty days of incubation at 27 ⁰C, in the dark, the enzymes were extracted in 50 mM sodium acetate buffer at pH= 5,4 using a shaker at 100 rpm for 1 h and enzymatic activities were determined. The strains produced different profiles of enzymatic activities. The higher hydrolytic activities (xylanase, xylosidase, carboxymethylcellulase, b-glucosidase) were obtained with the strains SJC and FEB-14, while the higher oxidative activities were obtained with the strains CCB-581 and CCB-515. Sawdust medium stimulated the hydrolytic enzymes, but the eucalypt residue produced approximately the same amount of enzymes. The use of L. edodes for biodegradation of eucalypt residue proved to be an efficient way to recycle this substrate.