Poster Presentation 1B-56
The Extracellular
Proteomes of Filamentous Fungi Grown on Complex Biomass
Ellen Panisko,
Scott Baker, Jon Magnuson and Linda Lasure*
Phone: (509) 375-2234
Fax: (509) 372-4732
E-mail: linda.lasure@pnl.gov
In order to hydrolyze the polysaccharides of complex biomass
to their constituent sugars, a variety of enzymes are required, and the filamentous
fungi are excellent producers of these enzymes. Many of the glycosyl
hydrolases and esterases are
known, but their regulation is complex. Understanding the hydrolytic enzymes
and their regulation will be crucial to improvement of fungi for the production
of enzymes or other products directly from biomass. The rapidly increasing
number of filamentous fungal genomes has created the opportunity to study fungi
using proteomics. Proteomics provides the tools to capture a picture of the
entire complement of proteins secreted by a fungus in response to a particular
biomass source. We have applied proteomics to the study of Trichoderma reesei and Phanerochaete chrysosporium (sequenced by the Joint
Genome Institute). Identifying the secreted proteins from these fungi promises
to increase our understanding of the process of lignocellulose
decomposition and help identify targets for improvement. Improved processes and
organisms will be critical for the development of integrated biorefineries.