Poster Presentation 1B-43

Effect of Media Composition and Growth Conditions on Production of

b-glucosidase by A. niger C-6

Oscar García-Kirchner,* Marcela Segura-Granados and Patricia Rodríguez-Pascual

Departamento de Bioprocesos

UPIBI-IPN. Av. Acueducto S/N Col. Barrio La Laguna, Ticomán.

México, 07340 D. F. MÉXICO

Phone: (5) 57-29-60-00 ext. 56345

Fax: (5) 57-29-60-00 ext. 56305

E-mail: ogarcia@acei.upibi.ipn.mx

Previously we reported liquid fermentation for b-glucosidase production with the strain of A. niger C-6 using shake flask culture in a basal culture medium and different lignocellulosic materials as the sole carbon source.

This presentation describes evaluation of the fermentation conditions: growth temperature, medium composition and pH for b-glucosidase production during six days’ growth using a simplified culture medium with C.S.L. and native sugar cane bagasse as the sole carbon source in a laboratory fermenter, working volume 1.0 litre, for the optimizing conditions.

The experimental data obtained indicate that productivity of b-glucosidase production by A. niger C-6 is a function of both temperature, pH and the cultivation medium. The maximum enzyme titer was obtained within 3 days. This results suggest that this strain is a potential microorganism for the scale up to a major level and further enzyme characterization.