Poster Presentation 1B-40

 

Utilization of Methyloleate in the Production of Yarrowia lipolytica Lipase

 

 

J. Destain,1* B. Moreau,1 F.Weekers,3 P. Fickers2 and P. Thonart1

 

 

1 Centre Wallon de Biologie Industrielle

Agricultural University of Gembloux, Belgium

Phone:  003281622311

Fax:  003281614222

E-mail:  destain.j@fsagx.ac.be

 

2 Centre Wallon de Biologie Industrielle, University of Liège, Belgium

 

3 Société Artechno, Belgium

 

 

 

Many microorganisms produce extracellular lipase, but only a few of these enzymes are commercially available: lipases from Aspergillus oryzae, Aspergillus niger, Rhizopus arrhizus, Rhizomucor miehei, Humicola lanuginosa.  The present work concerns the development of lipase from Yarrowia lipolytica.

 

A previous paper describes a mutant selection leading to high enzyme production (1000 Units/ml) in liquid medium containing glucose (1%), whey powder (3%), corn steep liquor (1% V/V), olive oil (0,5% V/V) and (NH4)2 SO4 (0,8 %).

 

In order to develop industrial media with low-cost raw materials, fatty acid ester has been introduced in the culture medium: methyloleate was first used successfully as an inducer in comparison to olive oil at the same concentration.  In a second approach we replaced one of the carbon sources of the medium (glucose) and the inducer (olive oil) with methyloleate.  Different concentrations from 0.5% to 5% were tested and compared to the reference.   Maximum activity (3000 Units/ml) is attained with 2,5% - 3 % of ester.  These results were confirmed in fermentors of different sizes: 2 liters, 20 liters and 500 liters.