Poster Presentation
1B-22
Partial
Purification and Characterization of Protease Enzyme from Bacillus subtilis megatherium Bacteria
Ayşe Gerze, Yüksel Güvenilir*
Istanbul Technical
University, Department of Chemical Engineering
80626, Maslak, Istanbul,
TURKEY
Phone: +90 212
2656294
E-mail:avcibasi@itu.edu.tr
The aim of
this experimental study is partial isolation and purification of protease
enzyme from Bacillus subtilis
megatherium bacteria species. Protease enzyme is
obtained by producing spore genesis of bacteria, which is from Bacillus Subtilis Megatherium species in suitable food places. The
purification was realized by applying respectively ammonium sulphate
precipitation, dialyse, DEAE-Cellulose ion exchange
chromatography to the supernatant that was produced later. In this study, the
effect of substrate concentration, reaction time, the effect of inhibitor and
activator on the optimum pH, optimum temperature, pH stability, temperature
stability was determined. Besides, molecular weight of the obtained enzyme was
investigated by realization of transaction of SDS-PAGE. Protease enzyme which
was purificated from Bacillus subtilis megatherium species, was purified 1.3 fold after ammonium sulphate precipitation.