Poster
Presentation 1B-13
Sugarcane
Bagasse Pulps Biobleached with Commercial Xylanase Cartazyme HS and with
Xylanase of Bacillus pumilus
1Departamento de
Biotecnologia, FAENQUIL
CP 116, CEP 12600-970 Lorena-SP,
Brazil
E-mail: adilson@debiq.faenquil.br
2Microbiology Laboratory,
CPQBA-UNICAMP, Campinas, Brazil
Ethanol/water pulping was performed
using ethanol/water 1:1 (v/v) at 185ºC for 2.5 h. Acetososlv pulping was performed
with a mixture of acetic acid and water 93% (v/v) and HCl as catalyst at 110°C
for 2 h. The pulps obtained were
biobleached with 18 UI per gram of dried pulp with Cartazyme and Bacillus pumilus for 4 h, 8 h and 12 h
at 50°C. The results of viscosity, kappa number and chemical composition of the
pulps are in Tables 1 and 2; the best results obtained were with 8 h of
treatment.
Table 1. Results of viscosity, kappa
number and chemical composition for ethanol/water pulps
|
Enzyme |
Treatment |
Viscosity (cP) |
Kappa Number |
Glucan (%) |
Xilan (%) |
Residual Lignin (%) |
|
Cartazyme |
X |
11,10 ± 0.26 |
38.35 ± 3.01 |
72.20 ± 0.68 |
6.79 ± 0.02 |
18.54 ± 1.54 |
|
XE |
20.09 ± 3.76 |
9.15 ± 0.16 |
87.83 ± 0.54 |
5.00 ± 0.06 |
6.51 ± 0.25 |
|
|
Bacillus pumilus |
X |
14.31 ± 5.70 |
42.68 ± 0.22 |
71.08 ± 1.81 |
8.76 ± 1.60 |
17.73 ± 1.10 |
|
XE |
13.12 ± 4.28 |
5.62
± 2.53 |
87.08 ± 0.76 |
7.11 ± 0.69 |
4.65 ± 1.35 |
X = Xylanase, XE = Xylanase followed
by alkaline extraction.
Table 2. Results of viscosity, kappa
number and chemical composition for acetosolv pulps
|
Enzyme |
Treatment |
Viscosity (cP) |
Kappa Number |
Glucan (%) |
Xilan (%) |
Residual Lignin (%) |
|
Cartazyme |
X |
5.50 ± 0.12 |
13.25 ± 3.07 |
61.27 ± 0.35 |
10.98 ± 0.08 |
20.67 ± 0.38 |
|
XE |
7.23± 0.15 |
8.33 ± 0.78 |
79.32 ± 3.51 |
9.41 ± 1.40 |
9.10 ± 3.35 |
|
|
Bacillus pumilus |
X |
6.70 ± 0.13 |
12.13 ± 3.60 |
73.29 ± 0.63 |
6.59 ± 1.42 |
14.98 ± 0.74 |
|
XE |
9.63 ± 0.13 |
8.54 ± 2.11 |
89.99 ± 0.72 |
3.81 ± 0.25 |
4.59 ± 0.80 |
X = Xylanase, XE = Xylanase followed
by alkaline extraction.
In ethanol/water and in acetosolv
pulps the treatment with xylanase of the B.
pumilus presented viscosity greater than that of the pulps treated with
Cartazyme. Acetosolv pulps treated with B.
pumilus showed residual lignin 27% lower than the pulps treated with
Catazyme.